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Karyotype and genome size analyses in species of Helichrysum (Asteraceae)

机译:蜡菊属植物(菊科)的核型和基因组大小分析

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Karyotype studies were performed in 18 populations of eight Helichrysum species in Iran. Those species showed chromosome numbers of 2n = 2x = 14; 2n = 4x = 24, 28 and 32; 2n = 6x = 36; 2n = 7x = 42; 2n = 8x = 48; 2n = 9x = 54; and 2n = 10x = 60. The chromosome numbers of H. davisianum, H. globiferum, H. leucocephalum and H. oocephalum are reported here for the first time. New ploidy levels are reported for H. oligocephalum (2n = 4x = 24) and H. plicatum (2n = 4x = 32). The chromosomes were metacentric and submetacentric. An ANOVA among H. globiferum and H. leucocephalum populations showed significant differences for the coefficient of variation for chromosome size, total form percentage and the asymmetry indices, indicating that changes in the chromosome structure of Helichrysum species occurred during their diversification. Significant positive correlations among the species and populations studied, in terms of the total chromosome length, lengths of the short arms and lengths of the long arms, indicate that these karyotypic features change simultaneously during speciation events. The genome sizes of Helichrysum species are reported here for first time. The 2C DNA content ranged from 8.13 pg (in H. rubicundum) to 18.4 pg (in H. leucocephalum and H. davisianum). We found that C-value correlated significantly with ploidy level, total chromosome length, lengths of the long arms and lengths of the short arms (p
机译:在伊朗的8种蜡菊属植物的18个种群中进行了核型研究。这些物种的染色体数目为2n = 2x = 14; 2n = 4x = 24、28和32; 2n = 6x = 36; 2n = 7x = 42; 2n = 8x = 48; 2n = 9x = 54;和2n = 10x =60。首次报道了戴维森菌,球茎杆菌,白头翁和头颅菌的染色体数。据报道,小头螺旋杆菌(2n = 4x = 24)和褶皱杆菌(2n = 4x = 32)有新的倍性水平。染色体是亚中心的和亚中心的。球茎H. leucocephalum种群之间的方差分析显示染色体大小,总形式百分比和不对称指数的变异系数存在显着差异,表明蜡菊属物种的染色体结构发生了变化。就总染色体长度,短臂长度和长臂长度而言,所研究的物种和种群之间存在显着的正相关性,表明这些核型特征在物种形成过程中同时发生变化。此处首次报道了蜡菊属物种的基因组大小。 2C DNA含量的范围从8.13 pg(在红宝石中)到18.4 pg(在白头翁和戴维斯犬中)。我们发现C值与倍性水平,总染色体长度,长臂长和短臂长(p

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