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首页> 外文期刊>Cytotechnology >Spermine enhances IgM productivity of human-human hybridoma HB4C5 cells and human peripheral blood lymphocytes
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Spermine enhances IgM productivity of human-human hybridoma HB4C5 cells and human peripheral blood lymphocytes

机译：精胺提高人-人杂交瘤HB4C5细胞和人外周血淋巴细胞的IgM生产率

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摘要

The polyamine spermine was assessed for enhancement of IgM production by human-human hybridoma, HB4C5 cells, under serum-free conditions. IgM production of HB4C5 cells was stimulated approximately 6-fold by the addition of 7.3 mM of spermine. The facilitating effect was observed soon after inoculation. In spite of suppression of cell growth, the IgM production rate was accelerated for at least 5 days without medium change. Moreover, laser confocal microscopic analysis revealed that the IgM content inside HB4C5 cells was increased by spermine treatment. These findings suggest that spermine enhances specific IgM productivity of the hybridoma line. Spermine also facilitated IgM production by human peripheral blood lymphocytes under serum-free conditions. This result implies that spermine enhances immunoglobulin production of not only specific hybridoma lines, but also non-specific immunoglobulin producers. Immunoglobulin production stimulating activity of spermine was accelerated 2-fold by the addition of DNA whith a chain length of about 400–7000 base pairs (bp). However, degraded short-chain DNA fragments (less than 200 bp) did not facilitate the immunoglobulin production stimulating activity of spermine.
机译：在无血清条件下，通过人-人杂交瘤HB4C5细胞评估了多胺精胺对IgM产生的增强作用。通过添加7.3 mM的精胺，HBgC5细胞的IgM产生被刺激了约6倍。接种后不久观察到促进作用。尽管抑制了细胞生长，但在不改变培养基的情况下，IgM的产生速度至少提高了5天。此外，激光共聚焦显微镜分析显示，通过精胺处理，HB4C5细胞内部的IgM含量增加。这些发现表明精胺增强了杂交瘤细胞系的特异性IgM生产力。在无血清条件下，精胺还促进人外周血淋巴细胞产生IgM。该结果暗示精胺不仅增强特异性杂交瘤细胞系的免疫球蛋白产生，而且还增强非特异性免疫球蛋白产生者的免疫球蛋白产生。通过添加链长约为400-7000个碱基对（bp）的DNA，精胺的免疫球蛋白生产刺激活性提高了2倍。但是，降解的短链DNA片段（小于200 bp）不能促进精胺的免疫球蛋白产生刺激活性。

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