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首页> 外文期刊>愛媛大学農学部紀要 >Effects of Polyamines on Proliferation and IgM Productivity of Human-human Hybridoma Cell Line
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Effects of Polyamines on Proliferation and IgM Productivity of Human-human Hybridoma Cell Line

机译：多胺对人-人杂交瘤细胞增殖和IgM生产力的影响

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摘要

Stimulating effects of polyamines on IgM producing human hybridoma cell line, HB4C5 cells were investigated. As the result of screening, several polyamines were found to facilitate IgM production of HB4C5 cells. Spermidine, thermine and triethylenetetraamine (TETA) stimulated IgM production by human-human hybridoma, HB4C5 cells, under the serum-free condition. IgM production of HB4C5 cells was accelerated 5.9-fold by spermidine, 5.3-fold by thermine, and 3.7-fold by TETA within 6 h after inoculation.Putrescine, however, did not enhance IgM production by HB4C5 cells at any dose. Spermidine enhanced IgM productivity of the hybridoma cells in spite of growth suppression. TETA inhibited cell proliferation and the remarkable effects on the accelerationof IgM productivity disappeared during 5 days because of its cytotoxicity. On the other hand, thermine facilitated IgM productivity of the hybridoma cells without growth suppression. The laser confocal microscopic analysis revealed that the IgM content inside HB4C5 cells was increased by thermine treatment. This result suggests that thermine provokes IgM synthesis of the hybridoma line.
机译：研究了多胺对产生IgM的人杂交瘤细胞株HB4C5细胞的刺激作用。筛选的结果是，发现了几种多胺可促进HB4C5细胞的IgM产生。在无血清条件下，亚精胺，嘧啶和三亚乙基四胺（TETA）刺激人-人杂交瘤HB4C5细胞产生IgM。接种后6小时内，亚精胺将HB4C5细胞的IgM产生促进了5.9倍，热胺将5.3倍，将TETA促进了3.7倍。尽管生长抑制，亚精胺提高了杂交瘤细胞的IgM生产率。 TETA由于具有细胞毒性，因此在5天之内就抑制了细胞增殖，并且对加速IgM生产力的显着影响消失了。另一方面，茶氨酸促进了杂交瘤细胞的IgM生产力，而没有生长抑制。激光共聚焦显微镜分析显示，乙胺嘧啶处理可增加HB4C5细胞内IgM含量。该结果表明，茶碱激发了杂交瘤细胞系的IgM合成。

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