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首页> 外文期刊>Acta biochimica Polonica >Lactococcus lactis IBB477 presenting adhesive and muco-adhesive properties as a candidate carrier strain for oral vaccination against influenza virus
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Lactococcus lactis IBB477 presenting adhesive and muco-adhesive properties as a candidate carrier strain for oral vaccination against influenza virus

机译：乳酸乳球菌IBB477具有粘附性和粘膜粘附性，可作为口服疫苗预防流感病毒的候选载体

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摘要

In the gastrointestinal tract (GIT), adhesion is a prerequisite for bacterial colonization. Lactococci can be used in functional food (probiotics) and health-related applications (mucosal vaccines, therapeutic drug delivery), both potentially involving adhesive properties. A candidate lactic acid bacterium for influenza antigen delivery through the GIT should display the ability to adhere. The present work probes the interactions between Lactococcus lactis and mucins using pig gastric mucin (PGM) as a model. Two strains were used for the optimization of the screening method for adhesion: L. lactis subsp. cremoris IBB477 persistent in the GIT of germ-free rats, and the low-adhering control strain MG1820. High adhesion to bare and mucin-coated polystyrene of IBB477 in comparison with MG1820 was observed. We searched for genetic determinants potentially involved in the adhesion/muco-adhesion of IBB477, identifying two such genes: prtP and a gene coding for a protein with MUB and MucBP domains. Based on its persistence in the GIT and adhesive properties, L. lactis IBB477 is a candidate carrier strain for expression of influenza haemagglutinin (HA) protein for induction of mucosal immune response.
机译：在胃肠道（GIT）中，粘附是细菌定植的前提。乳球菌可用于功能性食品（益生菌）和健康相关应用（粘膜疫苗，治疗性药物输送），两者都可能涉及粘合特性。通过GIT传递流感抗原的候选乳酸菌应具有黏附能力。本工作以猪胃粘蛋白（PGM）为模型探讨乳酸乳球菌和粘蛋白之间的相互作用。使用两种菌株来优化粘附力的筛选方法：乳酸乳杆菌亚种。 creemoris IBB477持续存在于无菌大鼠的GIT和低粘附力对照菌株MG1820中。与MG1820相比，观察到IBB477对裸露和粘蛋白涂层的聚苯乙烯的高附着力。我们搜索了可能与IBB477的黏附/粘膜黏附有关的遗传决定因素，从而确定了两个这样的基因：prtP和编码具有MUB和MucBP结构域的蛋白质的基因。基于其对GIT的持久性和粘附特性，乳酸乳杆菌IBB477是表达流感血凝素（HA）蛋白以诱导粘膜免疫反应的候选载体菌株。

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