One-step purification of vitronectin from human plasmaby affinity chromatography on phage-displayed peptides

Szymon Skurzynski

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One-step purification of vitronectin from human plasmaby affinity chromatography on phage-displayed peptides

机译：通过噬菌体展示肽的亲和层析从人血浆中一步纯化玻连蛋白

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A novel affinity purification method for rapid isolationof vitronectin (VN) from human plasma is described. Recentlywe have used phage display technology to obtainclones expressing peptides with high binding activity forVN. The isolated “strong VN binders” were covalentlycoupled to CNBr-activated Sepharose. Human plasmawas applied to the column and bound VN was elutedusing 0.5 M acetic acid, giving purity exceeding 90 %.The developed method is a convenient alternative toconventional antibody-antigen affinity chromatographytechniques for purification of VN, as it offers low ligandcost, is rapid and ensures good protein recovery fromhuman plasma.

机译：描述了一种从人血浆中快速分离玻连蛋白（VN）的新型亲和纯化方法。最近，我们已经使用噬菌体展示技术来获得表达对VN具有高结合活性的克隆。分离出的“强VN结合剂”与CNBr活化的琼脂糖共价偶联。将人血浆应用于色谱柱，并用0.5 M乙酸洗脱结合的VN，纯度超过90％。该开发的方法是常规抗体-抗原亲和层析技术纯化VN的便捷替代方法，因为它具有较低的配体成本，快速且可确保从人血浆中回收良好的蛋白质。

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《Acta biochimica Polonica》 |2010年第1期|共5页
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Szymon Skurzynski;
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1. One-step purification of vitronectin from human plasma by affinity chromatography on phage-displayed peptides [J] . Szymon Skurzynski Acta Biochimica Polonica . 2010,第Null期

机译：通过噬菌体展示肽的亲和层析从人血浆中一步纯化玻连蛋白
2. Novel Purification of Vitronectin from Human Plasma by Heparin Affinity Chromatography [J] . Heihachiro Kashiwagi, Masako Izumi, Masao Hayashi, Cell structure and function . 1988,第4期

机译：肝素亲和色谱从人血浆中纯化玻连蛋白
3. Selection of optimum affinity tags from a phage-displayed peptide library Application to immobilized copper(II) affinity chromatography [J] . A.V. Patwardhan, G.N. GouD, R.R. Koepsel, Journal of chromatography, A: Including electrophoresis and other separation methods . 1997,第1a2期

机译：从噬菌体展示的肽库中选择最佳亲和标记物应用于固定化铜（II）亲和色谱
4. Leukotriene-E4 in human urine: Comparison of on-line purification and liquid chromatography-tandem mass spectrometry to affinity purification followed by enzyme immunoassay [C] . Michael Armstrong, Andy Liu, Ronald Harbeck, ASMS Conference on Mass Spectrometry and Allied Topics . 2008

机译：人类尿液中的白酮-E4：在线纯化和液相色谱 - 串联质谱比较与亲和纯化，然后进行酶免疫测定
5. Purification of alpha-lactalbumin using peptides as affinity chromatography ligands. [D] . Gurgel, Patrick Vasconcelos. 2001

机译：使用肽作为亲和色谱配体纯化α-乳清蛋白。
6. Tagging Retrovirus Vectors with a Metal Binding Peptide and One-Step Purification by Immobilized Metal Affinity Chromatography [O] . Kaiming Ye, Sha Jin, Mohammad M. Ataai, 2004

机译：用金属结合肽标记一步病毒载体并通过固定金属亲和色谱法一步纯化
7. Tagging Retrovirus Vectors with a Metal Binding Peptide and One-Step Purification by Immobilized Metal Affinity Chromatography [O] . Ye, Kaiming, Jin, Sha, Ataai, Mohammad M., 2004

机译：用金属结合肽标记一步病毒载体并通过固定金属亲和色谱法一步纯化

One-step purification of vitronectin from human plasmaby affinity chromatography on phage-displayed peptides

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