...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta Biochimica Polonica >One-step purification of vitronectin from human plasma by affinity chromatography on phage-displayed peptides
【24h】

One-step purification of vitronectin from human plasma by affinity chromatography on phage-displayed peptides

机译：通过噬菌体展示肽的亲和层析从人血浆中一步纯化玻连蛋白

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A novel affinity purification method for rapid isolation of vitronectin (VN) from human plasma is described. Recently we have used phage display technology to obtain clones expressing peptides with high binding activity for VN. The isolated "strong VN binders" were covalently coupled to CNBr-activated Sepharose. Human plasma was applied to the column and bound VN was eluted using 0.5 M acetic acid, giving purity exceeding 90%. The developed method is a convenient alternative to conventional antibody-antigen affinity chromatography techniques for purification of VN, as it offers low ligand cost, is rapid and ensures good protein recovery from human plasma.
机译：描述了一种从人血浆中快速分离玻连蛋白（VN）的新型亲和纯化方法。最近，我们已经使用噬菌体展示技术来获得表达对VN具有高结合活性的肽的克隆。将分离的“强VN结合剂”共价偶联至CNBr活化的Sepharose。将人血浆应用于色谱柱，并用0.5 M乙酸洗脱结合的VN，纯度超过90％。所开发的方法是用于纯化VN的常规抗体-抗原亲和层析技术的便捷替代方法，因为它具有较低的配体成本，快速且可确保从人血浆中回收良好的蛋白质。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号