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首页> 外文期刊>Advanced Biomedical Research >Comparison of three phenotypic and deoxyribonucleic acid extraction methods for isolation and Identification of Nocardia spp
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Comparison of three phenotypic and deoxyribonucleic acid extraction methods for isolation and Identification of Nocardia spp

机译：三种表型和脱氧核糖核酸提取方法分离和鉴定诺卡氏菌的比较

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Background: The aerobic Actinomycetes are a large group of soil-indwelling bacteria that are distributed in world-wide. These Gram-positive bacteria are most commonly associated with opportunistic infections in both immunocompromised and immunocompetent hosts. Materials and Methods: In this study, three phenotypic and deoxyribonucleic acid (DNA) extraction methods for isolation and identification of Nocardia genus were compared. Samples were taken in five different locations of Isfahan's suburb from hospitals area, parks, agricultural lands, gardens, arid lands with different soil temperature and pH. Results: In this study, showed that slip-buried-method was better than two other phenotypic methods; 14 out of 70 soil samples (20%) were positive for Nocardia spp. DNA of positive samples were extracted with three techniques and DNA extraction by microwave technique was better than others. This technique was confirmed with observation of DNA bands on 1% agarose gel. Conclusions: These bacteria are important in immune deficient patients such as cancer patients, transplant recipients, tuberculosis; acquired immunodeficiency syndrome etc., Their affluence is unsteady in different zones of the world. In this study, among the three phenotypic methods for the isolation of Nocardia slip-buried method was better than other methods. Among DNA extraction techniques, DNA extraction by microwave method would be selective method for DNA extraction of Nocardia spp. compared with others techniques.
机译：背景：有氧放线菌是一大类土壤寄居细菌，分布在世界各地。这些革兰氏阳性细菌最常与免疫功能低下和免疫能力强的宿主中的机会性感染相关。材料与方法：本研究比较了三种分离和鉴定诺卡氏菌属的表型和脱氧核糖核酸（DNA）提取方法。在伊斯法罕郊区的五个不同地点分别从医院区域，公园，农业用地，花园，具有不同土壤温度和pH值的干旱土地中取样。结果：本研究表明，滑动掩埋法优于其他两种表型法; 70个土壤样品中有14个（20％）的诺卡氏菌呈阳性。用三种技术提取阳性样品的DNA，而用微波技术提取的DNA要好于其他技术。通过在1％琼脂糖凝胶上观察到DNA条带，证实了该技术。结论：这些细菌对免疫缺陷患者如癌症患者，移植受者，结核病很重要。后天免疫机能丧失综合症等，他们的富裕程度在世界不同地区并不稳定。在本研究中，用于分离诺卡氏菌的非表型的三种表型方法均优于其他方法。在DNA提取技术中，微波提取DNA是诺卡氏菌属DNA提取的选择性方法。与其他技术相比。

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