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首页> 外文期刊>Journal of medical bacteriology. >Isolation and Identification of Nocardia spp. Using Phenotypic Methods from Soil Samples of North Khorasan Province
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Isolation and Identification of Nocardia spp. Using Phenotypic Methods from Soil Samples of North Khorasan Province

机译：诺卡氏菌的分离与鉴定。使用表型方法从北呼罗珊省土壤样本中

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Introduction: Nocardia is saprophytic soil bacterium of the actinomycetes families. Nocardiahas high diversity of species and due to species diversity of Nocardia, phenotypic tests areessential for early identification of Nocardia species.Methods: 155 soil samples from different regions of North Khorasan province in Iran werecollected, including: urban and rural areas. Isolation of Nocardia was performed by paraffinbaiting Technique. Identification of Nocardia species was performed by phenotypic methodsthat are including: hydrolysis of the amino acids, acid production of carbohydrates and, growthat 35° C and 45°C.Results: 11 Nocardia species (7%) were identified . These bacteria were related to the gardens,the sands soils, and soil of town square. No growth was observed at 45°C. As the result, ourisolates were identified as Nocardia asteroides complex.Conclusion: Isolation and identification of Nocardia spp. from soil of different regions in NorthKhorasan province in Iran can help to enhance our understanding of epidemiological andecological of the pathogenic Nocardia species.
机译：简介：诺卡氏菌是放线菌科的腐生土壤细菌。诺卡氏菌的物种多样性很高，并且由于诺卡氏菌的物种多样性，表型测试对于诺卡氏菌的早期鉴定是必不可少的。方法：从伊朗北部霍拉桑省不同地区收集了155个土壤样品，包括城市和农村地区。诺卡氏菌的分离通过石蜡引诱技术进行。诺卡氏菌物种的鉴定采用表型方法，包括：氨基酸水解，糖类酸的产生以及在35°C和45°C下的生长。结果：鉴定出11种诺卡氏菌物种（7％）。这些细菌与花园，沙土和城镇广场的土壤有关。在45℃下未观察到生长。结果，我们的分离株被鉴定为诺卡氏菌小行星复合体。结论：诺卡氏菌的分离与鉴定。从伊朗北呼罗珊省不同地区的土壤中提取的盐可以帮助我们加深对致病诺卡氏菌物种的流行病学和生态学的了解。

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