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首页> 外文期刊>African Journal of Microbiology Research >Isolation and identification of secondary metabolites producer Nocardia spp. from Iraqi soil
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Isolation and identification of secondary metabolites producer Nocardia spp. from Iraqi soil

机译：次生代谢产物生产者诺卡氏菌的分离和鉴定。来自伊拉克

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One hundred and sixty nine Nocardia isolates were recovered from 111 Iraqi soil samples on six cultivation media, using paraffin baiting and dilution techniques. The North of Iraq soil was the richest in Nocardia. Paraffin baiting technique was more efficient in the isolation of Nocardia than dilution technique. Glucose asparagine agar was more suitable for cultivating Nocardia isolates followed by Sabouraud dextrose agar. All the 169 presumptive Nocardia isolates recovered were subjected to primary screening of antibacterial activity, with 10-55 mm inhibition zones against standard Staphylococcus aureus (NCTC 6571) and 10-38 mm inhibition zones against standard Escherichia coli (ATCC 25922). Eleven isolates with the highest antibacterial activities were selected for chemo-taxonomic studies. These isolates exhibited high similar features of 87-89% with Nocardia brasiliensis. Therefore, it is suggested to give proposed names for these new taxa: Nocardia sp.1, N. sp. 2, N. sp. 3, N. sp. 4, N. sp. 5, N. sp. 6, N. sp. 7, N. sp. 8, N. sp. 9, N. sp. 10 and N. sp. 11. .
机译：使用石蜡诱饵和稀释技术，从六种培养基上的111个伊拉克土壤样本中回收了169株诺卡氏菌。伊拉克北部的土壤是诺卡迪亚最丰富的土壤。石蜡鱼诱饵技术比稀释技术更有效地分离诺卡氏菌。葡萄糖天冬酰胺琼脂更适合用于培养诺卡氏菌分离株，然后是Sabouraud葡萄糖琼脂。回收的所有169种推定的诺卡氏菌菌株均经过了初步的抗菌活性筛选，具有针对标准金黄色葡萄球菌（NCTC 6571）的10-55 mm抑制区和针对标准大肠杆菌（ATCC 25922）的10-38 mm抑制区。选择了具有最高抗菌活性的11种分离物进行化学分类研究。这些分离物表现出与巴西诺卡氏菌高度相似的特征，为87-89％。因此，建议给这些新的分类群起建议的名称：诺卡氏菌属（Nocardia sp.1， 2，N。 3，N。 4，N. sp。 5，N。 6，N. sp。 7，N. sp。 8，N。 9，N。 10和N. sp。 11。

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