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首页> 外文期刊>Acta Universitatis Cibiniensis, Series E. Food Technology >Analysis on effect of separation and purification of glycoprotein extracted from Camellia seeds and its functional activity as basis for the economic development of Camellia oleifera industry
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Analysis on effect of separation and purification of glycoprotein extracted from Camellia seeds and its functional activity as basis for the economic development of Camellia oleifera industry

机译：油茶种子中糖蛋白的分离纯化效果及其功能活性作为油茶产业发展的基础

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Taking Camellia oleifera seeds as raw materials, this study explored extraction and purification of glycoprotein separated from Camellia seeds as well as its antitumor activity, aiming to provide a theoretical basis for the economic development of Camellia oleifera industry. Key impact factors of Camellia seed glycoprotein were extracted using buffer solution method and water extraction method and a regression model was set up. Methyl thiazolyl tetrazolium was used to evaluate the in vitro antitumor activity of glycoprotein extracted from Camellia seeds and Differential Scanning Calorimetry (DSC) was used to measure its denaturation enthalpy value. Results indicated that protein and sugar yields were 8.96% and 17.05% respectively under optimal conditions when water extraction method was used. Crude glycoprotein extracted from Camellia oleifera had a certain inhibitory effect on human hepatoma cell HepG2, gastric cancer cell MGC-803 and breast cancer cell MCF-7 and crude glycoprotein extracted from Camellia oleifera by water-extraction and alcohol-precipitation method had a strong antitumor effect. Crude glycoprotein obtained in the two different ways was capable of scavenging DPPH, ?OH and O2g- free radicals and also showed good reducing capacity. DSC measurement results revealed that specific rotation of COGP 2 a [ α ] n 20 was - 32.5. Antitumor experiment in vitro showed that glycoprotein extracted from Camellia seeds in the two different ways had a certain inhibitory effect on HepG2, MGC-803 and MCF-7, which has important theoretical and realistic significances to promoting utilization value of camellia resources, strengthening Camellia oleifera ’s comprehensive development and utilization of high added value as well as enriching types and functions of active glycoprotein.
机译：以油茶种子为原料，探讨了从油茶种子中分离得到的糖蛋白的提取纯化及其抗肿瘤活性，为油茶产业的经济发展提供理论依据。采用缓冲溶液法和水提取法提取山茶种子糖蛋白的关键影响因素，建立回归模型。甲基噻唑基四唑用于评估从茶花种子中提取的糖蛋白的体外抗肿瘤活性，并使用差示扫描量热法（DSC）来测量其变性焓。结果表明，在最佳条件下，采用水提法时蛋白质和糖的产率分别为8.96％和17.05％。油茶粗制糖蛋白对人肝癌细胞HepG2，胃癌细胞MGC-803和乳腺癌MCF-7有一定的抑制作用，油茶粗制糖蛋白通过水提醇沉法提取具有较强的抗肿瘤作用。影响。以两种不同方式获得的粗制糖蛋白能够清除DPPH，？OH和O 2 g-自由基，并具有良好的还原能力。 DSC测量结果表明，COGP 2a [α] n 20的比旋光度为-32.5。体外抗肿瘤实验表明，两种方法提取的茶花种子糖蛋白对HepG2，MGC-803和MCF-7有一定的抑制作用，对于提高茶花资源利用价值，增强油茶具有重要的理论和现实意义。公司的综合开发和利用具有高附加值，并丰富了活性糖蛋白的种类和功能。

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