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首页> 外文期刊>Advances in Life Sciences >The Improved Polymerase Chain Reaction Method Applied for Sex Identification of Crested Ibis, Nipponianippon
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The Improved Polymerase Chain Reaction Method Applied for Sex Identification of Crested Ibis, Nipponianippon

机译：改进的聚合酶链反应方法在凤头朱鹭的性别鉴定中的应用

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Given the necessity for increased conservation of endangered birds such as the Crested ibis, it is crucial to identify the gender of individuals from species with similar external morphologies among females(ZW) and males(ZZ) to establish a self-sustainable population for restoration of the species. Moreover, raising young chicks during the juvenile period based on sex determination encourages familiarity, which might increase mating success rates. Thus, this study was carried out to determine the sex of Crested ibis chicks using three primers designed to check their identity. A set of polymerase chain reaction(PCR) primers was used to amplify a 276 bp chromo-helicase-DNA binding protein region(CHD) on the Z chromosome in both sexes, whereas the other set was used to amplify a 169 bp female-specific CHD sequence on the W chromosome. The sequences have been submitted to NCBI(GenBank Accession Number: AB620020, AB620021). This method will enable researchers to determine the gender of Crested ibis more simply and quickly.
机译：鉴于有必要加强对凤头朱鹭等濒危鸟类的保护，从雌性（ZW）和雄性（ZZ）的具有相似外部形态的物种中识别个体的性别，以建立能够自我恢复的种群至关重要。该物种。此外，在幼年期根据性别决定饲养幼雏会促进熟悉度，这可能会增加交配成功率。因此，本研究使用三种设计用于检查其身份的引物来确定朱鹭的性别。一组聚合酶链反应（PCR）引物用于扩增男女两性Z染色体上的276 bp染色体-解旋酶-DNA结合蛋白区域（CHD），而另一组用于扩增169 bp的女性特异性W染色体上的CHD序列。该序列已经提交给NCBI（GenBank登录号：AB620020，AB620021）。这种方法将使研究人员能够更简单，更快速地确定朱rest的性别。

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