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Isolation, identification and evaluation of oil hydrocarbon decomposing bacteria from contaminated areas of oil fields

机译：油田污染区石油烃分解菌的分离鉴定与评价

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Microbialbiodegradation is known as an effective and harmless method to overcome environmental pollution with oil hydrocarbon. Some bacterial species were isolated from the Sarvestan oilfields (Iran, Fars province), then identified and applied for oil hydrocarbon decomposition. A carbon-free minimum medium (CFMM) containing 1% crude oil was used to isolate bacteria through incubation at 30°C in the dark at 200 rpm for 7 days. Different methods were used to identify the hydrocarbon oil decomposing bacteria: gram staining, squalene hydrolysis, catalase, production of arginine dihydrolase, gelatin liquefaction, hydrogen sulfide production, levan production, methyl red, oxidase, nitrite reduction, oxidative/fermentative, starch hydrolysis and Tween-80 hydrolysis tests. Nine different oil decomposing bacterial species were isolated. All the species grew well at 28 and 35°C, while four isolates containing of Bacillus sp. SA13, Pantoea sp. SA1112, Pseudomonas aeruginosa sp. SA21, and Bacillus sp. SA23 were capable of growing in a temperature of up to around 42°C. The minimum salt tolerance for isolates, except for Enterobacter sp. SA711, was 8%; Bacillus sp. SA212 had the highest tolerance of 15% sodium chloride. Acinetobacter sp. SA172, Enterobacter sp. SA711, Pseudomonas sp. SA75, Bacillus sp. SA212 and Bacillus sp. SA23 had the most growth rate in the CFMM. The highest percentages of oil removal obtained were 89% for Enterobacter sp. SA711, 86% for Acinetobacter sp. SA172, and 68% for Pseudomonas sp. SA75. The three isolated bacterial strains from the contaminated soil of the Sarvestan area had a good ability to degrade oil hydrocarbon. Therefore, they could be used commercially for the bioremediation of this region.
机译：微生物降解是克服石油烃污染环境的一种有效且无害的方法。从萨尔维斯坦油田（法尔斯省伊朗）分离出一些细菌，然后鉴定并用于石油烃分解。含有1％原油的无碳基本培养基（CFMM）用于通过在30°C下在黑暗中以200 rpm孵育7天来分离细菌。使用了不同的方法来鉴定分解烃油的细菌：革兰氏染色，角鲨烯水解，过氧化氢酶，精氨酸二水解酶的产生，明胶液化，硫化氢的产生，莱万的产生，甲基红，氧化酶，亚硝酸盐的还原，氧化/发酵，淀粉水解和Tween-80水解测试。分离出九种不同的油分解细菌物种。所有的菌种在28和35°C下均生长良好，同时含有4种分离的芽孢杆菌。 SA13，Pantoea sp。 SA1112，铜绿假单胞菌。 SA21和芽孢杆菌SA23能够在高达42°C的温度下生长。分离株的最低耐盐性，肠杆菌属除外。 SA711为8％;芽孢杆菌SA212具有最高的15％氯化钠耐受性。不动杆菌属SA172，肠杆菌属。 SA711，假单胞菌属。 SA75，芽孢杆菌属。 SA212和芽孢杆菌SA23在CFMM中的增长率最高。肠杆菌属的最高除油百分比为89％。 SA711，不动杆菌属的86％。 SA172，假单胞菌sp。占68％。 SA75。从萨尔维斯坦地区受污染的土壤中分离出的三种细菌菌株具有良好的降解石油烃的能力。因此，它们可商业用于该区域的生物修复。

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