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首页> 外文期刊>ACS Omega >Production and Characterization of Polyhydroxyalkanoate from Lignin Derivatives by Pandoraea sp. ISTKB
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Production and Characterization of Polyhydroxyalkanoate from Lignin Derivatives by Pandoraea sp. ISTKB

机译：Pandoraea sp。从木质素衍生物生产和表征多羟基链烷酸酯。 ISTKB

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The present study investigates polyhydroxyalkanoate (PHA) production from lignin and its derivatives by a previously reported lignin-degrading bacterial strain Pandoraea sp. ISTKB. PHA production was screened by fluorescence microscopy and flow cytometry using a Nile red stain. PHA and biomass accumulation, while screening, was found to be maximum on 4-hydroxybenzoic acid followed by p -coumaric acid, vanillic acid, 2,6-dimethoxyphenol, and kraft lignin after 96 h. Monomer composition was analyzed by gas chromatography–mass spectrometry (GC–MS) and was followed by Fourier transform infrared and ~(1)H NMR analysis, indicating PHA to be a copolymer of P(hydroxybutyrate-co -hydroxyvalerate). Genomic analysis of Pandoraea sp. ISTKB also complemented the results of GC–MS and NMR, and the relevant genes responsible for the synthesis of small chain length PHA were discovered in the genome. Process parameters were optimized by response surface methodology for enhanced production of PHA and biomass on 4-hydroxybenzoate. Optimization results showed 30 and 66% increase in the biomass and PHA production, respectively. The results obtained were promising and indicated that if lignin is depolymerized into low-molecular-weight intermediates, then it can easily be utilized and converted into value-added products like PHA by microbes.
机译：本研究调查了先前报道的降解木质素的细菌菌株潘多拉酵母菌（Pandoraea sp。）从木质素及其衍生物生产聚羟基链烷酸酯（PHA）的方法。 ISTKB。使用尼罗红染色，通过荧光显微镜和流式细胞术筛选PHA产生。在筛选的同时，发现PHA和生物质积累在96小时后在4-羟基苯甲酸上最大，其次是对-香豆酸，香草酸，2,6-二甲氧基苯酚和牛皮纸木质素。通过气相色谱-质谱法（GC-MS）分析单体组成，然后进行傅立叶变换红外光谱和〜（1）H NMR分析，表明PHA是P（羟基丁酸酯-共-羟基戊酸酯）的共聚物。 Pandoraea sp。的基因组分析。 ISTKB还补充了GC-MS和NMR的结果，并且在基因组中发现了负责小链长PHA合成的相关基因。通过响应表面方法优化工艺参数，以提高在4-羟基苯甲酸酯上生产PHA和生物质的能力。优化结果表明，生物量和PHA产量分别增加了30％和66％。所得结果令人鼓舞，表明如果将木质素解聚为低分子量中间体，则可以很容易地利用木质素并将其转化为增值产品，例如微生物产生的PHA。

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