The present study was aimed at the evaluation of the possible relationship between high temperature sand increased tolerance to water deficiency of Eucalyptus urophylla cell lineages on their physiological and biochemical aspects. For this purpose, calluses cultures derived from hypocotyls Eucalyptus urophylla were maintained in tissue culture in an N7 culture medium with 5 mg L -1 Picloram and used to conduct tests. The calluses were treated at different temperatures (25, 30, 35, 40 and 45°C) for 2 h in BOD and then subjected to a selection pressure in N7 medium supplemented with 20% polyethylene glycol (PEG 6000) for 20 days under constant light exposure (42 μmol m -2 s -1 PAR) and temperature (26±2°C) conditions with a 12 h photoperiod. For evaluate the experiment, proline, total soluble proteins, glucose, polyose and glucuronic acid contents were determined as well as the relative growth rate by measuring dry weight and fresh weight. The results indicated that previous treatment with higher temperatures may increase tolerance to drought stress. Therefore tolerant cell lines to stress conditions can be selected using this methodology.
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机译：本研究旨在评估在高温砂土对尾叶桉细胞谱系的水分缺乏耐受性的生理和生化方面的可能关系。为此，将源自下胚轴的尾叶桉（Eucalyptus urophylla）的愈伤组织培养物在具有5 mg L -1 Picloram的N7培养基中的组织培养物中进行保存，并用于进行测试。将愈伤组织在BOD中于不同温度（25、30、35、40和45°C）处理2 h，然后在补充有20％聚乙二醇（PEG 6000）的N7培养基中在恒定压力下经受选择压力20天。光照（42μmolm -2 s -1 PAR）和温度（26±2°C）条件下，光照时间为12小时。为了评估实验，通过测量干重和鲜重确定脯氨酸，总可溶性蛋白，葡萄糖，多糖和葡萄糖醛酸含量以及相对生长速率。结果表明，先前用较高温度处理可能增加对干旱胁迫的耐受性。因此，可以使用该方法选择对应激条件耐受的细胞系。
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