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首页> 外文期刊>ACS Omega >Red-Emissive Carbon Dots for “Switch-On” Dual Function Sensing Platform Rapid Detection of Ferric Ions and l-Cysteine in Living Cells
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Red-Emissive Carbon Dots for “Switch-On” Dual Function Sensing Platform Rapid Detection of Ferric Ions and l-Cysteine in Living Cells

机译：用于“接通”双功能传感平台的红色发光碳点可快速检测活细胞中的铁离子和半胱氨酸

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Ferric ions (Fe3+ ions) and l-cysteine (( l-Cys) in the human body have always played an irreplaceable role in biological processes, and overload or deficiency of Fe3+ ions and l-Cys in the biological system leads to various diseases. In this work, N,S-co-doped red-emitting carbon dots (R-CDs) were synthesized by a facile hydrothermal method. Because the doping of N and S gives a unique functional group distribution on the surface of R-CDs, it can be complexed with Fe3+ ions to construct an energy transfer quenching system. However, the presence of l-Cys competitively binds to Fe3+ ions, thus resulting in the photoluminescence recovery of R-CDs. Therefore, a “switch-on” dual function sensing platform has successfully been developed based on R-CDs for rapid identification and quantification of Fe3+ ions and l-Cys. The linear detection range of Fe3+ ions is 0–30 μM (limit of detection (LOD): 0.27 μM) and that of l-Cys is 0–24 μM (LOD: 0.14 μM). The sensor platform was used to detect Fe3+ ions and l-Cys in human serum samples with satisfactory results. Compared with traditional detection methods, this method is more time-saving and efficient and can be completed in 3 min. It is worth mentioning that the R-CDs not only has high optical stability but also has negligible cytotoxicity and has been successfully applied to in vitro/vivo imaging, indicating that R-CDs have excellent tissue penetration and biomarker potential. More interestingly, the switch-on fluorescence behavior for stepwise detection of Fe3+ ions and l-Cys can also be observed in cell imaging, which provides the possibility of visual detection of the probe to be applied in vivo.
机译：人体中的铁离子（Fe3 +离子）和l-半胱氨酸（（l-Cys）一直在生物过程中扮演着不可替代的角色，并且生物系统中Fe3 +离子和l-Cys的过量或缺乏会导致多种疾病。在这项工作中，通过简便的水热法合成了N，S掺杂的红色发光碳点（R-CD），由于N和S的掺杂在R-CD的表面具有独特的官能团分布，它可以与Fe3 +离子络合以构建能量转移猝灭系统，但是l-Cys的存在与Fe3 +离子竞争性结合，从而导致R-CD的光致发光恢复，因此具有“接通”双重功能基于R-CD的传感平台已成功开发，用于快速鉴定和定量Fe3 +离子和l-Cys，Fe3 +离子的线性检测范围为0–30μM（检测限（LOD）：0.27μM）， l-Cys为0–24μM（LOD：0.14μM），使用传感器平台检测Fe3 +血清样品中的离子和L-半胱氨酸离子，结果令人满意。与传统的检测方法相比，该方法省时，高效，可在3分钟内完成。值得一提的是，R-CDs不仅具有较高的光学稳定性，而且细胞毒性可忽略不计，并且已成功应用于体外/体内成像，表明R-CDs具有出色的组织渗透性和生物标志物潜力。更有趣的是，还可以在细胞成像中观察到逐步检测Fe3 +离子和l-Cys的开启荧光行为，这提供了可视检测探针在体内应用的可能性。

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