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首页> 外文期刊>ACS Omega >Potential Transfer of Peanut and/or Soy Proteins from Poultry Feed to the Meat and/or Eggs Produced
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Potential Transfer of Peanut and/or Soy Proteins from Poultry Feed to the Meat and/or Eggs Produced

机译：花生和/或大豆蛋白可能从家禽饲料转移到生产的肉和/或蛋中

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Previous studies have demonstrated that allergenic feed proteins from peanuts in the diets of layer hens are not detected in the eggs produced. Hence, in this study, we aimed to determine if soy and/or peanut proteins in poultry feed rations of broiler chickens or layer hens would be transferred or detectable in the meat or eggs produced. To meet this objective, 99 layer hens and 300 broiler chickens were equally divided into treatment groups and fed one of three experimental diets: control soybean meal and corn diet, whole unblanched high-oleic peanut and corn diet (HO PN), or a control diet spiked supplemented with oleic acid (OA) oil. At termination, broiler chickens were processed, and chicken breast samples of the left pectoralis muscle were collected, and eggs were collected from layers. Total protein extracts from pooled egg samples and chicken breast samples were subjected to enzyme-linked immunosorbent assay (ELISA) methods and immunoblotting analysis with rabbit antipeanut agglutinin antibodies and rabbit antisoy antibodies for the detection of peanut and soy proteins. Peanut and soy proteins were undetected in all pooled egg samples and individual chicken breast meat samples using immunoblotting techniques with rabbit antipeanut agglutinin and rabbit antisoy antibodies. Moreover, quantitative ELISA allergen detection methods determined all pooled egg samples and individual meat samples as “not containing” peanut or soy allergens. Therefore, this study helps to evaluate the risk associated with the potential transfer of allergenic proteins from animal feed to the products produced for human consumption.
机译：先前的研究表明，在蛋鸡中未检出花生中的过敏性饲料蛋白。因此，在这项研究中，我们旨在确定肉鸡或蛋鸡的家禽日粮中的大豆和/或花生蛋白是否会在生产的肉或蛋中转移或检测到。为了实现此目标，将99层母鸡和300只肉鸡平均分为治疗组，并饲喂以下三种实验饮食之一：对照豆粕和玉米饮食，全无油高花生和玉米饮食（HO PN）或对照饮食中加了油酸（OA）油。在终止时，处理肉鸡，并收集左胸大肌的鸡胸肉样本，并从各层收集鸡蛋。对来自合并卵样品和鸡胸肉样品的总蛋白提取物进行酶联免疫吸附测定（ELISA）方法，并用兔抗花生凝集素抗体和兔抗大豆抗体进行免疫印迹分析，以检测花生和大豆蛋白。使用兔抗花生凝集素和兔抗大豆抗体的免疫印迹技术，未在所有合并的鸡蛋样品和个别鸡胸肉样品中检测到花生和大豆蛋白。此外，定量ELISA过敏原检测方法将所有合并的鸡蛋样本和单个肉样本确定为“不含”花生或大豆过敏原。因此，这项研究有助于评估与变应原性蛋白质从动物饲料到生产供人类消费的产品的潜在转移有关的风险。

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