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首页> 外文期刊>ACS Omega >One-Step Fabrication of Enzyme-Immobilized Reusable Polymerized Microcapsules from Microfluidic Droplets
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One-Step Fabrication of Enzyme-Immobilized Reusable Polymerized Microcapsules from Microfluidic Droplets

机译:从微流控液滴一步一步制备酶固定的可重复使用的聚合微胶囊

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Enzyme immobilization is an essential prerequisite for biocatalysis. In this context, emulsion provides an excellent template for assembling enzymes at the oil–water interface. A microfluidic approach has been adopted to produce oil-in-water-type emulsions stabilized by gold nanoparticle–catalase conjugates. In situ ring-opening polymerization of the oil phase produces solid core enzyme-immobilized microcapsules (MCs). These resultant MCs exhibited a Km value of 42 mM and shows 1.1-fold higher activity compared to free enzymes. Finally, the robust MCs showed excellent recyclability, which can meet the demand of industrial biotechnological applications.
机译:固定酶是生物催化的必要前提。在这种情况下,乳液为在油水界面组装酶提供了极好的模板。已采用微流体方法来生产由金纳米颗粒-过氧化氢酶结合物稳定的水包油型乳液。油相的原位开环聚合产生固相酶固定的微胶囊(MCs)。与游离酶相比,这些生成的MC的Km值为42 mM,并且活性高1.1倍。最后,坚固的MC显示出出色的可回收性,可以满足工业生物技术应用的需求。

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