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首页> 外文期刊>African Journal of Biotechnology >Isolation and open reading frame 5 gene analysis of porcine reproductive and respiratory syndrome virus in Yunnan Province, China
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Isolation and open reading frame 5 gene analysis of porcine reproductive and respiratory syndrome virus in Yunnan Province, China

机译：云南省猪繁殖与呼吸综合征病毒的分离和开放阅读框5基因分析

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Two porcine reproductive and respiratory syndrome virus (PRRSV), respectively named YN-1 and YN-2 strains, were isolated by inoculation into Marc-145 cell. The two isolated strains induce Marc-145 cell stack together, pull net, form plaque and other typical lesions after 4 blind passages. With extracted viral RNA of fourth generation,?reverse transcriptase (RT)-PCR based on?open reading frame 5 (ORF5)gene showed that there was porcine reproductive and respiratory syndrome virus in Marc-145 cell of fourth generation. TCID50 of isolate measured by Reed-Muench method was 10-3.6/0.1 ml. Genetic evolution of ORF5 indicated that the two isolated strains were in a small branch with high identity of 99.5%. They were in a branch with Shandong strain JN-HS, Hennan-1 and Vietnam 347-T-KSA strain with identity of 99.2 to 99.8%. The two isolated strains were in a different branch with Ch-1a and VR-2332 strains having identity of 94.4 to 94.5%.
机译：通过接种到Marc-145细胞中，分离出两种分别命名为YN-1和YN-2株的猪繁殖与呼吸综合征病毒（PRRSV）。在4次盲传后，这两个分离的菌株共同诱导了Marc-145细胞堆叠，拉网，形成噬菌斑和其他典型病变。用提取的第四代病毒RNA，基于“开放阅读框5”（ORF5）基因的逆转录酶（RT）-PCR显示第四代Marc-145细胞中存在猪繁殖与呼吸综合征病毒。通过Reed-Muench方法测得的分离株的TCID50为10-3.6 / 0.1ml。 ORF5的遗传进化表明，两个分离的菌株位于一个小的分支中，具有高达99.5％的同一性。它们与山东菌株JN-HS，Hennan-1和越南347-T-KSA菌株在同一分支中，同一性为99.2至99.8％。两种分离的菌株与Ch-1a和VR-2332菌株在同一分支中，其同一性为94.4％至94.5％。

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