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首页> 外文期刊>African Journal of Biotechnology >The characterization of cytoplasmic ribosomal protein genes in microsporidian Nosema bombycis Genome
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The characterization of cytoplasmic ribosomal protein genes in microsporidian Nosema bombycis Genome

机译：微孢子虫Nosema bombycis基因组中胞质核糖体蛋白基因的表征

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Microsporidia are obligate intracellular, eukaryotic parasites of medical and commercial importance, which can infect almost all animals including humans. However, their ribosomes are not of the 80S type as other eukaryotes, but like the prokaryotic 70S ribosome. In order to get the global composition of ribosomal protein genes of Nosema bombycis, the pathogen of Pébrine, and their comparative genomics’ characteristics, a genome-wide survey in N. bombycis genome was performed. From the results, we identified 130 CDSs corresponding to 73 ribosomal protein genes. Among them, three ribosomal protein genes (RPL19, RPS4 and RPS18) with short introns (23 or 24 bps) were verified by N. bombycis ESTs, and they have the same structure among microsporidia. The novel arrangements of ‘AAATTT-like signal – CCC/GGG-like motif – transcription start site’ are present in the upstream sequences of ribosomal protein genes, and several regulatory elements that may have synergy with introns of ribosomal protein genes for its high transcriptional frequency were detected too. 76.7% ribosomal protein genes of N. bombycis were located in syntenic blocks, indicating that their gene order was conserved among microsporidian species. And phylogenic trees show its ancient eukaryotic position too. The characterization of the total ribosomal proteins contributes a first step to ribosomal proteins’ transcription regulation, evolution of microsporidia.
机译：微孢子虫是专一的细胞内真核生物，具有医学和商业意义，可感染几乎所有动物，包括人类。但是，它们的核糖体不像其他真核生物那样属于80S型，而是像原核的70S核糖体一样。为了获得Nosema bombycis的核糖体蛋白基因，Pébrine的病原体及其比较基因组学特征的核糖体蛋白基因的全球组成，对bombycis N.基因组进行了全基因组调查。从结果中，我们确定了对应于73个核糖体蛋白基因的130个CDS。其中，三个短内含子（23或24 bps）的核糖体蛋白基因（RPL19，RPS4和RPS18）通过N. bombycis EST验证，并且在小孢子虫中具有相同的结构。核糖体蛋白基因的上游序列中存在“ AAATTT样信号– CCC / GGG样基序–转录起始位点”的新颖排列，并且一些调节元件可能与核糖体蛋白基因的内含子协同作用，以实现其高转录度频率也被检测到。弹状猪笼草76.7％的核糖体蛋白基因位于同位块中，表明它们的基因顺序在小孢子虫物种中是保守的。系统发育树也显示出其古老的真核生物位置。总核糖体蛋白的表征是核糖体蛋白转录调控，小孢子虫进化的第一步。

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