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首页> 外文期刊>African Journal of Biotechnology >In vitro mass multiplication of pomegranate from cotyledonary nodal explants cv. Ganesh
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In vitro mass multiplication of pomegranate from cotyledonary nodal explants cv. Ganesh

机译：子叶节外植体cv石榴的体外大量繁殖。加内什

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摘要

For surface sterilization of explants, treatment involving HgCl2- (0.1 %) for 3 min gave better sterilization of cotyledonary nodal explants. The maximum percentage establishment of cotyledonary node explants was observed on Murashige and Skoog (MS) medium + 1.0 mg/l 6-benzylaminopurine (BAP) + 0.5 mg/l naphthaleneacetic acid (NAA). However, MS medium fortified with 1.0 mg/l BAP + 1.0 mg/l kinetin + 200 mg/l activated charcoal exhibited maximum multiplication rate for the first two subcultures. The maximum frequency of multiple shoots in cotyledonary explants (86.33 %) was observed on treatment MS + 1.0 mg/l BAP + 1.0 mg/l kinetin + 200 mg/l activated charcoal.?In vitro?rooting of regenerated shoot was found in half strength MS medium supplemented with 0.5 mg/l NAA + 200 mg/l activated charcoal, which recorded the maximum number of root/shoot (4.17) and root length (3.87 cm).In vitro?grown plantlets having 5 to 6 cm length of shoot were transferred to vermicompost + soil (1:1v/v) media kept in net house, which showed better survival of plantlet (85.50%) within 11.75 days.
机译：对于外植体的表面灭菌，使用HgCl2-（0.1％）进行3分钟的处理可以更好地对子叶节外植体进行灭菌。在Murashige和Skoog（MS）培养基+ 1.0 mg / l 6-苄氨基嘌呤（BAP）+ 0.5 mg / l萘乙酸（NAA）上观察到子叶节点外植体的最大建立百分比。但是，用1.0 mg / l BAP + 1.0 mg / l动蛋白+ 200 mg / l活性炭强化的MS培养基在前两个继代培养中表现出最大的繁殖率。在MS + 1.0 mg / l BAP + 1.0 mg / l激动素+ 200 mg / l活性炭处理下，子叶外植体多次芽的最大发生频率（86.33％）。补充0.5 mg / l NAA + 200 mg / l活性炭的强度MS培养基，记录的最大根数/枝数（4.17）和根长（3.87 cm）。将枝条转移到网箱中的house杆+土壤（1：1v / v）培养基中，在11.75天内显示出更好的存活率（85.50％）。

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