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Use of Saccharomyces cerevisiae and Zymomonas mobilis for bioethanol production from sugar beet pulp and raw juice

机译:酿酒酵母和运动发酵单胞菌用于从甜菜浆和原汁生产生物乙醇的用途

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Biofuels have received great attention as an alternative energy source mainly due to limited oil reserves. Bioethanol can be produced from wide range of raw materials like starch, sucrose and cellulosic based sources. Sugar beet and raw juice, as its intermediate product, constitute very profitable?substrates for bioethanol production, considering content of easy available fermentable?sugars. In this study, sugar beet pulp and raw juice were fermented with?Saccharomyces cerevisiae?distillery yeasts and bacterium?Zymomonas mobilis. Different medium dilution rate as well as yeasts preparations (Fermiol, Safdistil C-70) were investigated. Fermentation was run for 72 h at 30°C. Quality of obtained raw distillates was evaluated using GC method.?S. cerevisiae?distillery yeasts turned out to be more favourable microorganism than bacterium?Z. mobilis?for sucrose material fermentation. The ethanol yield obtained from sugar beet pulp and raw juice was 84 and 95% of theoretical yield, respectively. Fermentation of sugar beet raw juice obtained by pressing without enzymatic treatment yielded higher ethanol efficiency as compared to raw juice pressed with enzyme. Dilution ratio 1:1 for fermentation medium appeared to be profitable?for effective fermentation process.
机译:主要由于石油储量有限,生物燃料作为替代能源受到了广泛关注。生物乙醇可以由多种原料生产,例如淀粉,蔗糖和纤维素基原料。考虑到易得的可发酵糖的含量,甜菜和原汁作为其中间产品构成了非常有利可图的生物乙醇生产底物。在这项研究中,甜菜果肉和原汁分别用酿酒酵母,蒸馏酵母和细菌发酵单胞菌发酵。研究了不同的培养基稀释率以及酵母制剂(Fermiol,Safdistil C-70)。在30℃下发酵72小时。用GC法评价所得粗馏出物的质量。事实证明,酿酒酵母比酿酒酵母Z是更有利的微生物。 mobilis?用于蔗糖原料发酵。从甜菜浆和原汁中获得的乙醇产率分别为理论产率的84%和95%。与用酶压榨的原汁相比,未经酶处理的压榨获得的甜菜原汁的发酵产生更高的乙醇效率。对于有效的发酵过程,发酵培养基的1:1稀释比似乎是有利的。

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