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首页> 外文期刊>African Journal of Biotechnology >Pathogen detection and gut bacteria identification in Apis cerana indica in Thailand
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Pathogen detection and gut bacteria identification in Apis cerana indica in Thailand

机译：泰国Apis cerana indica的病原体检测和肠道细菌鉴定

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Pathogen infection of honeybees can lead to economic losses in apiculture. The earlier the pathogen contamination can be found the better it will likely be for the treatment of the infected colony and prevention of the spread of the pathogen within and between colonies. A total of 50 colonies of Apis cerana were sampled in Samut-Songkhram (five colonies) and Chumphon (45 colonies) provinces in the central and the south of Thailand, respectively. Diagnostic multiplex polymerase chain reaction (PCR) revealed that 20, 6, 4, 20 and 0% of the samples were infected by Paenibacillus larvae, Nosema ceranae, Nosema apis, Ascosphaera apis and Sacbrood virus (Morator aetatulus), respectively. Positive amplified PCR products of target genes were sequenced. A phylogenetic tree was constructed by the neighbor-joining (NJ) distance based, using the Phylogenetic Analysis Using Parsimony (PAUP 4.0b10) program. The phylogenetic relationship of each pathogen, based on the partial sequence of the 16S rRNA of P. larvae, N. ceranae and N. apis, and of the 5.8S rRNA of A. apis, revealed a significant difference from the non-Thai isolates of these pathogens, but no significant geographical isolation between the different Thai apiaries, although it separated some into closely related clusters. In order to reduce the use of antibiotics in an apiary, bacteria in the gut of healthy bees were focused. Interestingly, Bifidobacterium species, Lactobacillus species, Bacillus species, Lactobacillus spp. and other lactic acid bacteria, were isolated from larvae and adult workers, but gave conflicting preliminary identities based on their biochemistry-morphology versus sequence analysis of a partial fragment (1.4 kb) of their 16S rRNA.
机译：蜜蜂的病原体感染会导致养蜂业的经济损失。发现病原体污染的时间越早，治疗被感染菌落和防止病原体在菌落内和菌落之间扩散的可能性就越好。在泰国中部和南部的Samut-Songkhram（五个殖民地）和Chumphon（45个殖民地）省中，总共采样了Apis cerana的50个殖民地。诊断性多重聚合酶链反应（PCR）显示，分别有20％，6％，4％，4％，20％和0％的样本分别感染了Paenibacillus larvae幼虫，cerenae ceranae，Nosema apis，Ascosphaera apis和Sacbrood病毒（Moator aetatulus）。对靶基因的正扩增PCR产物进行测序。使用近亲系统发育分析（PAUP 4.0b10）程序，根据相邻连接（NJ）距离构建系统发育树。每种病原体的系统发育关系基于幼虫，蜡状猪笼草和apis的16S rRNA的部分序列，以及apis的5.8S rRNA的部分序列，揭示了与非泰国分离株的显着差异。尽管这些病原体将某些病原体分成了紧密相关的簇，但它们之间并没有明显的地理隔离。为了减少在蜂房中使用抗生素，对健康蜜蜂肠道中的细菌进行了研究。有趣的是，双歧杆菌属，乳杆菌属，芽孢杆菌属，乳杆菌属。从幼虫和成年工人中分离出了其他乳酸菌和其他乳酸菌，但根据它们的生化形态与16S rRNA部分片段（1.4 kb）的序列分析得出的初步身份相互矛盾。

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