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首页> 外文期刊>African Journal of Biotechnology >Genetic variability of indigenous cowpea genotypes as determined using inter-simple sequence repeats markers
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Genetic variability of indigenous cowpea genotypes as determined using inter-simple sequence repeats markers

机译：使用内部简单序列重复标记确定土著cow豆基因型的遗传变异

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Cowpea [Vigna unguiculata (L.) Walp.] is cultivated widely by small farmers in the semiarid region of Northeastern Brazil for subsistence purposes, especially to complement the family income. However, owing to the limited availability of water in this region, there is an urgent need for novel highly productive drought-tolerant cultivars. The aim of the present study was to establish the genetic variability of 14 cowpea populations (60 indigenous genotypes from 13 microregions of Rio Grande do Norte and 4 domesticated cultivars produced by Embrapa) using inter-simple sequence repeats (ISSR) markers. The set of 13 selected primers generated a total of 257 loci, 247 (96.11%) of which were polymorphic, with sizes ranging between 200 and 2000 bp. Genetic similarities between accessions were estimated from Jaccard coefficients and genetic relationships were determined from the dendrogram constructed using the unweighted pair group method with arithmetic average (UPGMA) technique. Bayesian statistics coupled with the Markov chain Monte Carlo technique was applied to determine population structure, while the genetic variability was established by analysis of molecular variance. UPGMA analysis allowed the separation of the genotypes into three groups, but no relationship between the genetic and geographical distances was observed. The fixation index was considered intermediary (FST = 0.0818), the average heterozygosity was low (HS = 0.39) and the coefficient of endogamy was high (f = 92.6%). The results show the presence of genetic diversity among the studied populations and revealed that such variability could be attributed mainly to intra-population variability (91.82%).
机译：pea豆[Vigna unguiculata（L.）Walp。]由巴西东北半干旱地区的小农广泛种植，以维持生计，特别是为了增加家庭收入。但是，由于该地区水的供应有限，迫切需要新型的高产耐旱品种。本研究的目的是使用简单重复序列（ISSR）标记建立14个cow豆种群的遗传变异性（来自北里奥格兰德州13个微区的60个土著基因型和由Emrapa生产的4个驯化品种）。这组13个选定引物共产生257个基因座，其中247个（96.11％）是多态的，大小在200至2000 bp之间。利用Jaccard系数估算种质间的遗传相似性，并使用算术平均（UPGMA）技术通过非加权对群方法，通过树状图确定遗传关系。贝叶斯统计结合马尔可夫链蒙特卡罗技术被用来确定种群结构，而遗传变异性是通过分子变异分析来确定的。 UPGMA分析允许将基因型分为三类，但是没有观察到遗传距离与地理距离之间的关系。固视指数被认为是中间的（FST = 0.0818），平均杂合度低（HS = 0.39），内婚系数高（f = 92.6％）。结果表明，研究种群之间存在遗传多样性，并且表明这种变异性主要归因于种群内变异性（91.82％）。

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