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首页> 外文期刊>African Journal of Biotechnology >The inhibitory effect of Lactobacillus sakei KBL isolated from kimchi on the adipogenesis of 3T3-L1 cells
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The inhibitory effect of Lactobacillus sakei KBL isolated from kimchi on the adipogenesis of 3T3-L1 cells

机译：泡菜中分离出的日本乳酸杆菌KBL对3T3-L1细胞成脂的抑制作用

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Abnormal adipocyte growth, in terms of increased cell numbers and increased cell differentiation, is considered to be a major pathological feature of obesity. Thus, the inhibition of preadipocyte mitogenesis and differentiation could help prevent and suppress obesity. Some probiotics and cellular components are known to modulate the lipid metabolism in vitro and/or in vivo. The aim of this study was to investigate whether extracts from Lactobacillus sakei KBL cells isolated from kimchi could exert anti-adipogenic action in 3T3-L1 cells (fat cells). Differentiating 3T3-L1 cells were treated with L. sakei KBL cell extracts (L. sakei KBL_CE), and cell viability was assessed by MTT assays. At concentrations below 1 mg/ml, L. sakei KBL_CE did not exert any cytotoxic effect in 3T3-L1 cells. Lipid regulation in the cell culture system was assessed by morphological analysis and oil-red-O staining of fat. Treatment with L. sakei KBL_CE significantly inhibited adipocyte differentiation. L. sakei KBL_CE treatment (1 mg/ml) also reduced lipid accumulation by 25% in fully differentiated 3T3-L1 adipocytes. These findings collectively indicate that L. sakei KBL_CE can reduce fat mass by modulating adipogenesis in maturing preadipocytes.
机译：就增加的细胞数量和增加的细胞分化而言，异常的脂肪细胞生长被认为是肥胖症的主要病理特征。因此，抑制前脂肪细胞的有丝分裂和分化可以帮助预防和抑制肥胖。已知一些益生菌和细胞成分在体外和/或体内调节脂质代谢。这项研究的目的是调查从泡菜中分离出的日本乳酸杆菌KBL细胞的提取物是否可以在3T3-L1细胞（脂肪细胞）中发挥抗脂肪形成作用。分化的3T3-L1细胞用日本乳杆菌KBL细胞提取物（日本乳杆菌KBL_CE）处理，并通过MTT分析评估细胞活力。在低于1 mg / ml的浓度下，日本乳杆菌KBL_CE对3T3-L1细胞没有任何细胞毒性作用。通过脂肪的形态分析和油红O染色评估细胞培养系统中的脂质调节。清酒乳杆菌KBL_CE处理可显着抑制脂肪细胞分化。清酒乳杆菌KBL_CE处理（1 mg / ml）在完全分化的3T3-L1脂肪细胞中也使脂质蓄积减少了25％。这些发现共同表明，日本乳杆菌KBL_CE可通过调节成熟的前脂肪细胞中的脂肪生成来减少脂肪量。

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