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首页> 外文期刊>African Journal of Biotechnology >Screening and cloning of differentially expressed genes in Dendrobium nobile induced by orchid mycorrhizal fungus promoting the growth
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Screening and cloning of differentially expressed genes in Dendrobium nobile induced by orchid mycorrhizal fungus promoting the growth

机译：兰花菌根真菌促进生长的铁皮石end中差异表达基因的筛选与克隆

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Appropriate mycorrhizal fungi could effectively promote plant growth and development. Our previous research results showed that the growth of?Dendrobium nobile?was obviously promoted under inoculating one orchid mycorrhizal fungi,?Epulorhiza?sp. AR-18. To understand the growth-promoting molecular mechanisms, differential displayed real time polymerase chain reaction (DDRT-PCR), reverse Northern blot and Southern blot were used to isolate and identify genes whose transcription were altered in cultured?D. nobile?plants that were treated with?Epulorhiza?sp. AR-18. Amplified by 8 primer combinations from one anchor primers and 8 random primers, a total of 14 complementary?DNA?(cDNA) fragments including 12 differentially expressed cDNA bands were isolated. Reverse northern blot analysis showed that only 2 genes were differentially displayed cDNA bands. One band was an especially expressed fragment, expressed in the treated group but not in the control; while another was a differentially expressed fragment, weak in the treated and strength in the control. Southern blot analysis demonstrated that the two gene fragments were from the plant and not from the fungus.?Sequence analysis and database searches revealed no significant homology to any known sequences. The results suggested that the usefulness of messenger?RAN?(mRNA) differential display technique for the detection of differentially expressed genes in?D. nobile?whose growth could be promoted by mycorrhizal fungi.
机译：适当的菌根真菌可以有效促进植物的生长发育。我们以前的研究结果表明，接种一种兰花菌根真菌“ Epulorhiza” sp后，“金D石？”的生长明显得到了促进。 AR-18。为了了解促进生长的分子机制，使用差异显示实时聚合酶链反应（DDRT-PCR），反向Northern印迹和Southern印迹来分离和鉴定在培养的D中转录被改变的基因。用Epulorhiza？sp处理过的高贵植物。 AR-18。用1个锚定引物和8个随机引物的8个引物组合扩增，总共分离出14条互补的DNA片段，包括12条差异表达的cDNA条带。反向Northern印迹分析显示只有2个基因差异显示了cDNA条带。一个条带是特别表达的片段，在治疗组中表达，但在对照组中不表达;而另一个是差异表达的片段，在治疗上较弱，在对照中则较弱。 Southern印迹分析表明，这两个基因片段来自植物而不是真菌。序列分析和数据库搜索显示与任何已知序列均无显着同源性。结果表明，信使RAN？（mRNA）差异显示技术可用于检测？D中差异表达的基因。菌根真菌可促进其生长。

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