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首页> 外文期刊>African Journal of Biotechnology >Occurrence of Bacillus thuringiensis in faeces of herbivorous farm animals
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Occurrence of Bacillus thuringiensis in faeces of herbivorous farm animals

机译：草食性牲畜粪便中苏云金芽孢杆菌的发生

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摘要

Bacillus thuringiensis?(Berliner), the insect pathogen has been isolated from a variety of habitat. It is understood that the habitat of?B. thuringiensis?has always been associated with their biological activity. In the present study,?B. thuringiensiswas isolated from faeces of cows and goats. The phenotypic characterization revealed that the?B. thuringiensis?isolates are motile and harbour bipyramidal and spherical crystals, which was confirmed by the electron microscopic studies. The biochemical characterization confirmed the haemolytic nature and nitrate reduction ability of the?B. thuringiensis?isolates. There is a distinct variation in protein profiles among the isolates as revealed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. From this study we hypothesize that application of cattle manure in addition to maintaining the soil health also help augment?B. thuringiensis?population in soil, as a natural biocontrol agent and may play a role in the biological nitrogen cycle.
机译：苏云金芽孢杆菌（Berliner）是一种昆虫病原体，已从多种生境中分离出来。据了解，乙的栖息地。苏云金芽孢杆菌一直与其生物学活性有关。在本研究中，？苏云金从牛和山羊的粪便中分离出来。该表型特征表明。苏云金芽孢杆菌的分离物是能动的并带有双锥体和球形晶体，这已通过电子显微镜研究得到证实。生化特征证实了βB的溶血性质和硝酸盐还原能力。苏云金芽孢杆菌。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分析显示，分离物中的蛋白质谱存在明显差异。从这项研究中，我们假设除了保持土壤健康外，施用牛粪肥还有助于增加B。苏云金芽孢杆菌作为自然生物防治剂在土壤中的种群，并可能在生物氮循环中起作用。

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