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首页> 外文期刊>African Journal of Biotechnology >Characterization of stem rust resistance gene Sr2 in Indian wheat varieties using polymerase chain reaction (PCR) based molecular markers
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Characterization of stem rust resistance gene Sr2 in Indian wheat varieties using polymerase chain reaction (PCR) based molecular markers

机译：基于聚合酶链反应（PCR）的分子标记表征印度小麦品种的茎锈病抗性基因Sr2

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Stem rust or black rust is one of the most important diseases of wheat worldwide. In India, central, peninsular and southern hill zones are particularly prone to stem rust where favourable environmental conditions exist. The recent emergence of wheat stem rust race Ug99 (TTKSK) and related strains threatens global wheat production as Ug99 overcome resistance gene?Sr31 effective for many years. Resistance geneSr2, derived from cultivar ‘Hope’ is responsible for slow rusting and providing partial but durable resistance against stem rust of wheat. In addition to other unknown minor genes (Sr2 complex), this gene?tends to be non-specific and is currently effective against all isolates of?Puccinia graminis tritici?throughout wheat-growing regions of the world.? A set of 135 bread wheat varieties developed in the last forty years for prominent northern, central, peninsular and southern hill regions of India?wasscreened with molecular markers, CsSr2 and GWM533, developed and identified forSr2 gene. Out of 135 varieties screened, 92 confirmed the presence of?Sr2 gene at molecular level.?The molecular information of?Sr2?gene was corroborated with the available morphological marker data for selected varieties to evaluate the efficacy of these molecular markers in Indian wheat germplasm.?It was observed that these two molecular markers in combination provide accuracy in 92% lines for this gene at molecular level with presumed?Sr2 status in Indian wheat varieties. It is proposed that the use of?CsSr2 and GWM533 will help in gene pyramiding of?Sr2 along with other stem rust resistance genes in future wheat varieties to accelerate Indian breeding program for rust resistance.
机译：茎锈或黑锈是全世界小麦最重要的疾病之一。在印度，如果存在有利的环境条件，中部，半岛和南部丘陵地区特别容易发生铁锈病。由于Ug99克服了许多有效的抗性基因？Sr31，最近出现的小麦茎锈病Ug99（TTKSK）及其相关菌株威胁着全球小麦生产。源自“ Hope”品种的抗性基因Sr2负责缓慢生锈，并提供对小麦茎杆锈病的部分但持久的抗性。除其他未知的次要基因（Sr2复合体）外，该基因倾向于是非特异性的，目前对全世界小麦种植地区的所有小麦单胞菌分离株均有效。在过去的四十年中，针对印度北部，中部，半岛和南部丘陵地区开发的一套135个面包小麦品种进行了筛选，并用分子标记CsSr2和GWM533进行了筛选，并鉴定了Sr2基因。在筛选出的135个变种中，有92个在分子水平上确认了Sr2基因的存在。通过选择的可用形态标记数据证实了Sr2基因的分子信息，以评估这些分子标记在印度小麦种质中的功效。观察到，这两种分子标记的结合在该基因的分子水平提供了92％品系的准确性，并且在印度小麦品种中具有假定的Sr2地位。建议在未来的小麦品种中使用？CsSr2和GWM533有助于？Sr2以及其他抗茎锈基因的基因金字塔化，以加快印度的抗锈育种计划。

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