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首页> 外文期刊>African Journal of Biotechnology >In vitro evaluation of antioxidant activity of Anisopus mannii N.E. Br.
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In vitro evaluation of antioxidant activity of Anisopus mannii N.E. Br.

机译：拟南芥（Anisopus mannii N.E.） Br。

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Anisopus mannii?(Asclepiadaceae) –a plant widely used as an anti-diabetic agent in Northern Nigerian traditional medicine, was subjected to antioxidant evaluation. The crude methanol extract,?n-butanol and ethyl acetate fractions were investigated for free radical scavenging activity of the 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH), total phenolic contents and reducing power assay. The results of the DPPH free radical scavenging at 250 μgml-1?indicated maximum antioxidant activity of 94.1, 94.3 and 88.7% for crude methanol extract, ethyl acetate and?n-butanol fractions, respectively. The total phenolic content was higher in the ethyl acetate fraction (59.60 mg/g gallic acid equivalent GAE) than in the?n-butanol fraction (56.40 mg/g GAE) and crude methanol extract (53.20 mg/g GAE). The reducing power of?n-butanol fraction was 0.112 nm higher than those of ethyl acetate fraction (0.110 nm) and crude methanol extract (0.106 nm). The antioxidant activities of the crude extract/fractions and the standards at maximum concentration of 250 μgml-1decreased in the order: ascorbic acid> ethyl acetate fraction> crude methanol extract> Gallic acid>?n-butanol fraction, but there was no significant difference (p < 0.05) between them. The phytochemical screening revealed the presence of flavonoids, tannins and saponins. This suggests a potential utility of the plant as a source of phenolic antioxidants and may provide leads in the ongoing search for natural antioxidants from Nigerian medicinal plants to be used in treating diseases related to free radical reactions.
机译：尼日利亚北部传统药物中广泛用作抗糖尿病药物的曼尼古斯曼阿尼索斯（Asisopis mannii）科植物接受了抗氧化剂评估。研究了粗甲醇提取物，正丁醇和乙酸乙酯馏分的1,1-二苯基-2-吡啶并二肼基自由基（DPPH）的自由基清除活性，总酚含量和还原能力测定。 250μgml-1？清除DPPH自由基的结果表明，粗甲醇提取物，乙酸乙酯和正丁醇馏分的最大抗氧化活性分别为94.1、94.3和88.7％。乙酸乙酯级分（59.60 mg / g没食子酸当量GAE）中的总酚含量高于正丁醇级分（56.40 mg / g GAE）和粗甲醇提取物（53.20 mg / g GAE）。正丁醇馏分的还原能力比乙酸乙酯馏分（0.110 nm）和粗甲醇提取物（0.106 nm）的还原能力高0.112 nm。最高浓度为250μgml-1时，粗提取物/馏分和标准品的抗氧化活性依次降低：抗坏血酸>乙酸乙酯馏分>粗甲醇提取物>没食子酸>？正丁醇馏分，但无显着差异（p <0.05）之间。植物化学筛选显示了类黄酮，单宁和皂苷的存在。这表明该植物作为酚类抗氧化剂的来源具有潜在的实用性，并且可以为从尼日利亚药用植物中寻找用于治疗与自由基反应有关的疾病的天然抗氧化剂提供线索。

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