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首页> 外文期刊>African Journal of Biotechnology >The effect of plant growth regulators on optimization of tissue culture system in Malaysian upland rice
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The effect of plant growth regulators on optimization of tissue culture system in Malaysian upland rice

机译：植物生长调节剂对马来西亚陆稻组织培养系统优化的影响

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Mature seeds of four upland rice cultivars namely Kusan, Lamsan, Selasi and Siam were assessed for callus induction and plant regeneration on different concentrations and combinations of plant growth regulators, incorporated into MS (Murashige and Skoog) basal medium. Callus induction frequency was significantly different among the cultivars, as well as among the 2,4-Dichlorophenoxyacetic acid (2,4-D) levels tested. All tested cultivars exhibited highest callus frequency at 2 mgl-1?2,4-D. The incorporation of α-naphthaleneacetic acid (NAA) and kinetin (Kin) in the callus induction medium supplemented with 2 mgl-1?2,4-D did not significantly improve the callus induction frequency. After two subcultures, at 24 days interval, the best response to callus induction was from cultivar Selasi, while callus browning became prominent in cultivars Kusan and Siam.?Embryogenic callus placed on different regeneration media exhibited the highest regeneration frequency on medium containing 0.5 mgl-1?NAA + 2.0 mgl-1?Kin + 2.0 mgl-1?6-benzylaminopurine (BAP). The maximum regeneration frequency was achieved in cultivar Selasi followed by Lamsan while Siam and Kusan exhibited poor regeneration response. Among the four upland rice cultivars evaluated, Selasi and Lamsan are two promising cultivars in terms of callus induction frequency and morphology, and regeneration ability of the embryogenic callus.
机译：评估了四个旱稻品种（Kusan，Lamsan，Selasi和Siam）的成熟种子在不同浓度和植物生长调节剂组合下的愈伤组织诱导和植株再生，并结合到MS（Murashige和Skoog）基础培养基中。各品种之间以及2,4-二氯苯氧基乙酸（2,4-D）水平之间的愈伤组织诱导频率显着不同。所有测试的品种在2 mgl-1？2,4-D处表现出最高的愈伤组织频率。在添加了2 mgl-1？2,4-D的愈伤组织诱导培养基中掺入α-萘乙酸（NAA）和激动素（Kintin）不会显着提高愈伤组织的诱导频率。在两次传代培养后，每隔24天，对愈伤组织诱导的最佳响应来自Selasi品种，而愈伤组织褐变在Kusan和Siam品种中尤为明显。置于不同再生培养基上的胚性愈伤组织在含有0.5 mgl- 1 -NAA + 2.0 mgl-1-激酶+ 2.0 mgl-1 -6-苄基氨基嘌呤（BAP）。 Selasi和Lamsan达到了最大的再生频率，而Siam和Kusan表现出较差的再生响应。在评估的四个陆地稻品种中，就愈伤组织的诱导频率和形态，以及胚性愈伤组织的再生能力而言，Selasi和Lamsan是两个很有前途的品种。

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