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Colchicine and amiprophos-methyl (APM) in polyploidy induction in banana plant

机译:秋水仙碱和甲胺磷(APM)在香蕉植物多倍体诱导中的作用

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The objective was to assess the colchicine and amiprophos-methyl (APM) concentration and exposure period in the chromosome duplication of breed banana plants diploids.? Banana stem tips were used from the following genotypes: breed diploids (1304-04 [Malaccensis x Madang (Musa acuminata spp. banksii)] and 8694-15 [0337-02 (Calcutta x Galeo) x SH32-63]). ?Colchicine was used at concentrations of 0 (control treatment), 1.25, 2.5 and 5.0 mM, while APM was used at 0 (control treatment), 40 and 80 μM, in solution under agitation (20 rpm), for 24 and 48 h periods. With the use of APM, 66.67% tetraploid plants were obtained in the 1304-04 genotype using 40 μM for 24 h and 18.18% in 80 μM for 48 h, while in the 8694-15 genotype using 40 and 80 μM colchicine for 48 h, 27.27 and 21.43% tetraploid plants were observed, respectively.? For colchicine, in the 1304-04 genotype, only the 1.25 mM treatment for 48 h presented 25% tetraploid plants and in the 8694-15 genotype, the 5.0 mM concentration for 48 h produced 50% tetraploid plants. APM for 24 h enabled the tetraploid plant of the 1304-04 genotype to be obtained, while colchicine for 48 h resulted in tetraploid plants in the 8694-15 genotype.
机译:目的是评估香蕉品种二倍体染色体复制中秋水仙碱和氨甲基苯丙氨酸(APM)的浓度和暴露时间。使用了以下基因型的香蕉茎尖:繁殖二倍体(1304-04 [Malaccensis x Madang(Musa acuminata spp。bankii)]和8694-15 [0337-02(Calcutta x Galeo)x SH32-63])。在搅拌(20 rpm)的溶液中分别以0(对照处理),1.25、2.5和5.0 mM的浓度使用α秋水仙碱,同时以40和80μM在0(对照处理)的浓度下使用APM 24和48 h期。通过使用APM,在1304-04基因型中使用40μM进行24 h获得了66.67%的四倍体植物,在80μM中48.18%的获得了18.18%的基因,而在使用40和80μM秋水仙碱进行48 h的8694-15基因型中获得了。分别观察到27.27%和21.43%的四倍体植物。对于秋水仙碱,在1304-04基因型中,只有1.25 mM处理48 h才能显示25%的四倍体植物,而在8694-15基因型中,5.0 mM浓度持续48 h则能产生50%的四倍体植物。 APM 24 h能够获得1304-04基因型的四倍体植物,而秋水仙碱48 h产生8694-15基因型的四倍体植物。

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