• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >Monechma ciliatum methanolic extract regulates low density lipoprotein receptor and 3-hydroxy-3-methylglutaryl coenzyme A reductase genes expression in HepG2 cells
【24h】

Monechma ciliatum methanolic extract regulates low density lipoprotein receptor and 3-hydroxy-3-methylglutaryl coenzyme A reductase genes expression in HepG2 cells

机译：Monechma纤毛甲醇提取物调节HepG2细胞中的低密度脂蛋白受体和3-hydroxy-3-methylglutaryl辅酶A还原酶基因的表达

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Monechma ciliatum?methanolic extract (MCME) obtained from?Monechma ciliatumseedcake?showed high total phenolic compounds with high antioxidant activity.?The regulatory effects of MCME at 10, 20 and 50 μg/ml on low density lipoprotein receptor (LDLR) and 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR) were investigated in human HepG2 cell line using quantitative real-time polymerase chain reaction. LDLR mRNA level was increased significantly by 1.4, 2.6 and 4.3 fold in MCME treated cells at 10, 20 and 50, respectively, compared to untreated cells. Whereas, HMGCR mRNA level was decreased significantly by 38, 63 and 80% in MCME treated cells at 10, 20 and 50, respectively, compared to untreated cells. The effect of MCME was concentration dependent, and different doses showed significant differences in regulation of both LDLR and HMGCR genes. The present study showed that MCME effectively regulated the expression of LDLR and HMGCR genes influencing the cholesterol? metabolism in HepG2 cells.
机译：从“ Monechma纤毛种子蛋糕”获得的Monechma纤毛甲醇提取物（MCME）显示出高的总酚类化合物，具有很高的抗氧化活性。MCME对10、20和50μg/ ml的调节作用对低密度脂蛋白受体（LDLR）和3-使用定量实时聚合酶链反应在人类HepG2细胞系中研究了羟基3-甲基戊二酰辅酶A还原酶（HMGCR）。与未处理的细胞相比，在MCME处理的细胞中，LDLR mRNA水平分别以10、20和50分别显着增加了1.4倍，2.6倍和4.3倍。相比之下，与未处理的细胞相比，MCME处理的细胞的HMGCR mRNA水平分别下降了38％，63％和80％（分别为10、20和50）。 MCME的作用是浓度依赖性的，并且不同剂量在LDLR和HMGCR基因的调控上均表现出显着差异。本研究表明MCME有效调节了影响胆固醇的LDLR和HMGCR基因的表达。 HepG2细胞中的新陈代谢。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号