• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >Production and partial characterization of an exopolysaccharide from Ustilago maydis in submerged culture
【24h】

Production and partial characterization of an exopolysaccharide from Ustilago maydis in submerged culture

机译：浸没培养物中玉米Us的外多糖的生产和部分表征

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In Mexico, corn is?widely cultivated and?frequently parasitized by?Ustilago?maydis, abasidiomycete?dimorphic fungus?that causes?the disease known as?corn smut.?It iswidely?consumed?and is?considered?a culinary delight.?From a?biotechnologicalperspective,?U.?maydis?offers the?possibility as a?producer of?various metabolites, such as?laccase?and tyrosinase?enzymes,?glycolipids, and?indole derivatives?that can be used?in pharmaceutical,?cosmetic and?food, due?to its safety. Theexopolysaccharides (EPS)?are?extracellular?sugar?compounds?synthesized by avariety of microorganism?and have shown?various properties?and applications.?Among them are the?improved?rheology, texture,?stability and?mouthfeel?of fermented dairy products. In addition,?these compounds?have shown?biological activity?as an antitumor agent, immune-stimulating?and?reducing?blood cholesterol. The production of EPS byU. maydis?was studied by culturing the cells in yeast extract-peptone-dextrose (YPD), YPD supplemented with CuSO4?(YPDT), Czapek Dox-sucrose, Czapek Dox-glucose, Czapeck Dox culture media with corn steep liquor and in another media containing high oil oleic sunflower as the sole carbon source. The presence of activity of glycosyltransferase by?sodium dodecyl sulfate-polyacrylamide gel electrophresis (SDS-PAGE)?was detected as well as the polymers formed by dinitrosaliscilic acid (DNS). The products of fermentation were characterized by enzymatic hydrolysis using carbohydrases, gel permation chromatography and atomic force microscopy.
机译：在墨西哥，玉米被广泛种植，并经常被引起乌斯梯亚哥（Estilago）maydis的双歧真菌双态真菌所寄生，该病导致被称为玉米黑穗病。玉米被广泛食用，被认为是一种烹饪的乐趣。从生物技术的角度来看，美国大学可能会提供各种代谢物的生产商，例如，可用于药物的各种酶，例如“漆酶”和“酪氨酸酶”酶，“糖脂”和“吲哚衍生物”。由于其安全性，“化妆品和食品”。外多糖（EPS）是由多种微生物合成的细胞外糖化合物，具有多种特性和应用。其中，发酵乳制品的流变性，质地，稳定性和口感得到了改善。产品。此外，这些化合物已显示出具有生物活性，可以作为抗肿瘤剂，刺激免疫力和减少血液中的胆固醇。由U生产的EPS。通过在酵母提取物-蛋白糊-葡萄糖（YPD），补充有CuSO4？（YPDT）的YPD，Czapek Dox-蔗糖，Czapek Dox-葡萄糖，Czapeck Dox培养基，玉米浆和其他培养基中培养细胞来研究maydis？含有高油酸油葵作为唯一碳源。检测到十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）检测到的糖基转移酶活性以及由二亚硝基水杨酸（DNS）形成的聚合物。发酵产物通过使用糖酶的酶促水解，凝胶渗透色谱法和原子力显微镜来表征。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号