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首页> 外文期刊>African Journal of Biotechnology >Micropropagation of Caralluma stalagmifera var. longipetala: A rare succulent medicinal plant from Karnataka, India
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Micropropagation of Caralluma stalagmifera var. longipetala: A rare succulent medicinal plant from Karnataka, India

机译：Caralluma stalagmifera var。的微繁殖。 longipetala：来自印度卡纳塔克邦的稀有肉质药用植物

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An efficient in vitro protocol has been developed for the multiplication of shoots and conservation of a rare succulent medicinal plant Caralluma stalagmifera var. longipetala growing wildly in Karnataka State. Proliferation of multiple shoots was achieved on Murashige and Skoog’s (MS) medium supplemented with various concentrations of 6-benzyladenine (BA), Kinetin (Kn), indole acetic acid?(IAA), α- naphthalene acetic acid (NAA) and indole-3-butyric acid (IBA) alone or with various combinations from the nodal explants. The nodal explants cultured on medium containing BAP (2.0 mg/L) along with 0.5 mg/L Kn and 0.3 mg/L IAA produced the highest number of shoot sprouting (2.60±0.16) and maximum shoot length (3.96±0.20). The considerable frequency of callus induction and embryogenesis was noticed both in 1.0 mg/L NAA and 0.5-2 mg/L, 2, 4-D. The calli transferred to shoot induction medium containing the combination of hormones BAP (1.0 mg/L) plus IAA (0.2 mg/L) and NAA (0.1 mg/L) successfully regenerated in vitro shootlets. The in vitro rooting was achieved from both direct shoots regenerated from nodal explants and callus derived shootlets with NAA (0.2 mg/L). The in vitro rooted plantlets were successfully acclimatized (75%) in the greenhouse and gradually transferred to open field conditions.?Key word: Micro propagation, medicinal plant, Caralluma stalagmifera var. longipetala.
机译：已经开发了一种有效的体外方案，用于繁殖芽和稀有多汁药用植物石竹Caralluma stalagmifera var的保存。 longipetala在卡纳塔克邦疯狂生长。在补充了各种浓度的6-苄腺嘌呤（BA），激动素（Kn），吲哚乙酸？（IAA），α-萘乙酸（NAA）和吲哚- 3-丁酸（IBA）单独使用或与节点外植体组合使用。在含有BAP（2.0 mg / L）以及0.5 mg / L Kn和0.3 mg / L IAA的培养基上培养的结节外植体产生最高的芽萌芽数（2.60±0.16）和最大的芽长（3.96±0.20）。在1.0 mg / L的NAA和0.5-2 mg / L的2，4-D中均观察到愈伤组织的诱导和胚发生的频率。将愈伤组织转移到含有激素BAP（1.0 mg / L）加IAA（0.2 mg / L）和NAA（0.1 mg / L）的组合的芽诱导培养基中，可以成功地在体外再生小芽。从节点外植体再生的直接芽和用NAA（0.2 mg / L）的愈伤组织衍生的芽中获得了体外生根。体外生根的小苗在温室中成功地适应了环境（75％），并逐渐转移到露天条件下。关键词：微繁殖，药用植物，石竹（Caralluma stalagmifera var）。 longipetala。

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