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ISOLATION OF AN ANTI-FALLIPHARUM DRUG CANDIDATE FROM THE LEAUES OF INDIAN ETHOS -MEDICINAL PLANT BAN-NARINGA OR SUREGADA MULTIFLORA (SYNOSMY; GELONIUM MULTIFLOROM)
ISOLATION OF AN ANTI-FALLIPHARUM DRUG CANDIDATE FROM THE LEAUES OF INDIAN ETHOS -MEDICINAL PLANT BAN-NARINGA OR SUREGADA MULTIFLORA (SYNOSMY; GELONIUM MULTIFLOROM)
Leaves extract from Indian folk medicinal plant, ban-naringa effective against pali- azar or malaria, was shown to have anti-malarial activities against Plasmodium falcipharum in vitro. A 25% aquous-ethanolic crude extract (KS) inhibited 59% of the parasite growth in human RBC cells at 1 % concentration. Concentrated Fraction Y or TLC purified phyto-chemicals or most purified bioactive compound, KS-2000 completely killed the parasites in vitro at a concentration 5-10μg/ml. Cytocidal activities were assayed by both 3H-Hypoxanthine incorporation and Flurometric assays using Artimisinin and Chloroquine as standard. Crude extract or partially pure phytochemical did not inhibit bacterium Escherichia, coli DH5∞, human HeLa cells or molly fishes but significantly active against other protozoan parasites of the genus Leishmania and Trypanosoma at 20ug/ml and 50ug/ml respectively. The result demonstrated for the first time that ban-naringa plant (Scientific name: Suregada multiflora and Synosym: Gelonium multiflorum) may be used as a potential source to develop broad spectrum anti-protozoal drug to cure Bengal"s pali-azar and kala-azar or new world"s sleeping-sickness. The HPLC, MS, NMR, UV spectra(s) of KS-2000 were initiated for identity the novel active principle, different from known active principles. Mosquito-driven malaria disease is now spreading worldwide due to unavailability of vaccine and chloroquine resistance. Further investigation on KS- 2000 may provide a lead to develop a better toxic drug against human blood borne protozoa.
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