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Assessment of genetic diversity of rice (Oryza sativa) cultivars using ssr markers

机译:利用SSR标记评估水稻(Oryza sativa)品种的遗传多样性

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A set of 36 polymorphic simple sequence repeat (SSR) primers well distributed on all the 12 rice chromo-somes have been used to assess the genetic diversity among the rice varieties. A total of 98 alleles were detected with an average of 2.78 alleles per locus across 39 genotypes.? The number of alleles varied from two to four of these SSR loci. Among the primers, RM 401, RM 20A, RM 536, RM 7575 and RM 5862 produced maximum number of alleles (4). Out of the 98 alleles, 86 were polymorphic and this exhibited 87.76% polymorphism. SSR marker analysis differentiated the genotypes into two distinct groups. The polymorphism information content (PIC) value of the markers ranged between 0.28 and 0.50 with a mean value of 0.45. The highest PIC value (0.50) was observed for ten primers that is, RM 2, RM 24, RM 411, RM 104, RM 266, RM 125, RM 4674, RM 247, RM 3476 and RM 3351 while the lowest was 0.28 for RM 29. The genetic similarity coefficients for 39 genotypes obtained with SSR markers ranged from 23.8 to 78.4%.
机译:一组分布在所有12个水稻染色体上的36个多态性简单序列重复(SSR)引物已用于评估水稻品种之间的遗传多样性。总共检测到98个等位基因,其中39个基因型的每个基因座平均有2.78个等位基因。这些SSR基因座的等位基因数量从2个到4个不等。在这些引物中,RM 401,RM 20A,RM 536,RM 7575和RM 5862产生的等位基因数量最多(4)。在98个等位基因中,有86个是多态性的,表现出87.76%的多态性。 SSR标记分析将基因型分为两个不同的组。标记的多态信息含量(PIC)值介于0.28和0.50之间,平均值为0.45。十个引物的最高PIC值(0.50)为RM 2,RM 24,RM 411,RM 104,RM 266,RM 125,RM 4674,RM 247,RM 3476和RM 3351,而最低的为0.28。 RM 29.用SSR标记获得的39个基因型的遗传相似系数在23.8%至78.4%之间。

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