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首页> 外文期刊>African Journal of Biotechnology >Molecular cloning and phylogenetic analysis of a novel BURP domain-containing gene from Camellia sinensis
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Molecular cloning and phylogenetic analysis of a novel BURP domain-containing gene from Camellia sinensis

机译：茶树新的含BURP结构域基因的分子克隆和系统进化分析

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摘要

BURP domain-containing proteins are?a new class of?plant-specific proteins thatplay important roles in the growth, development and stress response of plants. In this paper, a novel?BURP domain-containing gene from?Camellia sinensis?(CsBDP,GenBank accession No. EU715397) was isolated and cloned using reverse transcription-polymerase chain reaction (RT-PCR)?followed by 5′ and 3′ rapid amplification of cDNA ends (RACE) reactions.?Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)?and Western blot were then applied to detect the expression of?CsBDP?gene in?Escherichia coli. Sequence comparison showed that the?deduced protein sequence of?CsBDP?gene shared high sequence similarity to other BURP domain-containing proteins and contained four typical modules: an N-terminal hydrophobic region, a short conserved segment, a variable region containing two?repeats?of about 19?amino acids,?which is unique for the RD22-like proteins of BURP family and a C-terminal BURP domain. Furthermore, phylogenetic tree revealed that the BURP protein family can be classified into eight subfamilies, and CsBDP was distinctly clustered into subfamily III with all RD22-like proteins. Therefore,?CsBDP?gene might be a stress-inducible gene and important for the stress tolerance of tea plant.
机译：含BURP结构域的蛋白质是一类新型的植物特异性蛋白质，在植物的生长，发育和胁迫响应中起着重要作用。本文通过逆转录-聚合酶链反应（RT-PCR）技术分离并克隆了一个新的来自中国茶树的含BURP结构域的基因（CsBDP，GenBank登录号EU715397），然后依次克隆了5'和3'。然后用十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）和蛋白质印迹法检测大肠杆菌中CsBDPβ基因的表达。序列比较表明，推导的CsBDP基因的蛋白序列与其他含BURP结构域的蛋白具有高度的序列相似性，并包含四个典型的模块：一个N端疏水区，一个短的保守区段，一个包含两个重复的可变区。大约19个氨基酸，对于BURP家族的RD22样蛋白和C端BURP域而言是独特的。此外，系统进化树显示，BURP蛋白家族可分为8个亚家族，而CsBDP与所有RD22样蛋白均明显地聚类为III家族。因此，CsBDP基因可能是胁迫诱导基因，对茶树的抗逆性具有重要意义。

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