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The influence of carbon source and calcium on the production of proteases by Erwinia chrysanthemi

机译：碳源和钙对菊花欧文氏杆菌产生蛋白酶的影响

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摘要

Erwinia chrysanthemi?(Ec) is the causative agent of soft rot diseases in many plants. In order to cause disease, it produces hydrolytic enzymes, which are involved in the degradation of plant tissue. However, the role of proteases in its pathogenicity to plants is not well understood.?E. chrysanthemi?targets the host’s middle lamella, which was mainly made of pectic substances containing calcium, for colonization. In this study, it is reported that a pectic substance, such as sodium polypectate (NaPP) may be preferable, not only for the production of hydrolytic enzymes, but for the production of protease as well. Furthermore, since the middle lamella contains high levels of calcium, it was thought that it may play an important role in the production of proteases by this organism. Both predictions were supported by the results presented in this study. When?E. chrysanthemi?subsp.?chrysanthemi?(Ecc) was grown in HXC medium supplemented with NaPP and calcium, it produced the highest levels of protease as compared to NaPP alone. These results suggest that proteases produced by?E.?chrysanthemi?may be induced in part by calcium.
机译：欧文氏菊（Erwinia chrysanthemi？）是许多植物中软腐病的病原。为了引起疾病，它产生水解酶，其参与植物组织的降解。但是，蛋白酶在其对植物的致病性中的作用尚不十分清楚。 chrysanthemi？将寄主的中间薄片定居，该中间薄片主要由含有钙的果胶物质制成。在该研究中，据报道，不仅对于水解酶的产生，而且对于蛋白酶的产生，诸如聚果酸钠（NaPP）的果胶物质也可能是优选的。此外，由于中间薄片含有高水平的钙，因此认为它可能在该生物体产生蛋白酶中起重要作用。两项预测均得到本研究结果的支持。什么时候？在补充NaPP和钙的HXC培养基中生长chrysanthemi？subsp。？chrysanthemi？（Ecc），与单独使用NaPP相比，它产生的蛋白酶水平最高。这些结果表明，大肠杆菌可能产生的蛋白酶部分地被钙诱导。

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