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首页> 外文期刊>African Journal of Biotechnology >Application of inter simple sequence repeat (ISSR marker) to detect genotoxic effect of heavy metals on Eruca sativa (L.)
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Application of inter simple sequence repeat (ISSR marker) to detect genotoxic effect of heavy metals on Eruca sativa (L.)

机译：间简单重复序列（ISSR标记）在检测重金属对紫草的遗传毒性作用中的应用

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As an influence of the Mediterranean diet,?Eruca sativa?(rocket salad) is eaten all over the world in salads and soups. It belongs to plant order Capparales (glucosinolate-containing species) and it is from the family Brassicaceae. Predominantly, the leaves of this species is eaten raw or cooked, although flowers are also consumed. Assessment of environmental contamination on ecology (plant) at molecular and population levels is important in risk quantification and remediation study. Heavy metal toxicity in plants is to induce oxidative stress linked to oxidation of proteins and membrane lipids but also to alterations of DNA damage response.?E. sativa?has been investigated in our study which is of agronomical importance and widely used in European countries. We studied three heavy metals Zn, Pb and Cd which showed a dose-dependent effect on radicle and coleoptile lengths of?E. sativa. The radicle length was more affected than the coleoptiles length under all concentration tested plant. The ranking of genotoxic potencies in all three heavy metals was in the descending order: Cd2+?> Pb2+?≥ Zn2+. Among these heavy metals, high concentration of Cd (150 mg/l) and Pb (150 mg/l) generated mutations along with changed morphology of seedlings. The radicle and coleoptile lengths (cm) under high concentration of?Cd?were decreased as compared to low, medium and high concentrations treated seedlings with Pb and Zn. 20?ISSR primers were used, of which four did not amplify, three gave single band and the rest of thirteen primers generated upto six bands (an average of 4 bands per primer). Sixteen primers exhibiting amplified products gave monomorphic; only two primers (OPC-5 and OPC-7) gave unique extra band in seedlings treated with medium and high concentrations of heavy metals Cd and Pb, respectively. The dendrogram was constructed to evaluate the genetic distance generated among the seedling treated with various heavy metals at various concentrations. The similarity matrix values were found from 42.8 to 100% and these values showed the genetic divergence among the seedlings treated with various concentrations of heavy metals.
机译：作为地中海饮食的一种影响，沙拉和汤在全世界范围内都食用“芝麻菜”（火箭沙拉）。它属于植物类Capparales（含芥子油苷的物种），来自十字花科。该物种的叶子主要是生食或煮熟的，尽管花朵也被消耗。在分子和种群水平上对生态（植物）环境污染的评估在风险量化和补救研究中很重要。植物中的重金属毒性是诱导与蛋白质和膜脂质氧化有关的氧化应激，也与DNA损伤反应的改变有关。在我们的研究中对苜蓿进行了研究，这具有重要的农艺意义，并已在欧洲国家广泛使用。我们研究了三种重金属Zn，Pb和Cd，它们对E的胚根和胚芽鞘长度显示出剂量依赖性。苜蓿。在所有受试植物中，胚根长度比胚芽鞘长度受到的影响更大。在所有三种重金属中，遗传毒性的等级从高到低依次为：Cd2 +→Pb2 +→≥Zn2+。在这些重金属中，高浓度的Cd（150 mg / l）和Pb（150 mg / l）会引起突变以及幼苗形态发生变化。与低，中和高浓度铅和锌处理过的幼苗相比，高浓度的Cd？下的胚根和胚芽鞘长度（cm）减小了。使用20？ISSR引物，其中4个未扩增，3个给出单条带，其余13个引物产生多达6个条带（每个引物平均4个条带）。展示扩增产物的十六个引物具有单态性;在分别用中等浓度和高浓度重金属Cd和Pb处理的幼苗中，只有两种引物（OPC-5和OPC-7）给出了独特的额外带。构造树状图以评估用各种浓度的各种重金属处理的幼苗之间产生的遗传距离。发现相似度矩阵值在42.8％至100％之间，这些值显示了在不同浓度的重金属处理下的幼苗之间的遗传差异。

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