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首页> 外文期刊>African Journal of Biotechnology >Detection and partial identification of proteins in pearls formed in Hyriopsis cumingii (Lea)
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Detection and partial identification of proteins in pearls formed in Hyriopsis cumingii (Lea)

机译：三角帆蚌（Lea）中形成的珍珠中蛋白质的检测和部分鉴定

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摘要

Pearl and nacre (mother of pearl) have similar chemical compositions. However, more than 20 proteins have been identified in nacre, yet none have been detected in pearl thus far. This study aimed to detect and identify protein in pearl. Two batches of pearls formed in?Hyriopsis cumingii?(Lea) were purchased from two pearl farms. They were ground into a powder of >10,000 mesh followed by ultra-sonication and extraction in water for 4 h at room temperature. The solution was centrifuged and the supernatant was saved as pearl powder water extract. A portion of the extract was heated at 121°C for 20 min. TCA precipitation and tricine–SDS-PAGE were conducted on both the heated and non-heated extracts. After silver nitrate staining, the heated extract demonstrated a distinct protein signal, but the non-heated extract did not. The protein band from each of the two heated extracts was excised from the gel and subjected to tryptic digestion and RP-nano-HPLC-ESI-MS/MS analysis. A MASCOT search of the results indicated that one protein had significant sequence homology to a putative vitelline envelop receptor for lysine in the common marine mussel?Mytilus edulis, and the other to the putative imaginal disc growth factor (IDGF) of?Diaprepes?abbreviatus.
机译：珍珠和珍珠母（珍珠母）具有相似的化学组成。然而，珍珠母中已鉴定出20多种蛋白质，但迄今为止在珍珠中均未检出。这项研究旨在检测和鉴定珍珠中的蛋白质。从两个珍珠养殖场购买了两批在“三角丁香”中形成的珍珠。将它们研磨成> 10,000目的粉末，然后进行超声波处理，并在室温下于水中提取4小时。将溶液离心，并将上清液保存为珍珠粉水提取物。将一部分提取物在121°C加热20分钟。 TCA沉淀和tricine-SDS-PAGE在加热和未加热的提取物上进行。硝酸银染色后，加热的提取物显示出明显的蛋白质信号，但未加热的提取物则没有。从凝胶上切下两个加热提取物中每一个的蛋白带，并进行胰蛋白酶消化和RP-nano-HPLC-ESI-MS / MS分析。对结果的MASCOT搜索表明，一种蛋白质与常见的海洋贻贝Mytilus edulis中的假定的卵黄素信封包裹的赖氨酸具有显着的序列同源性，而另一种蛋白质与Diaprepes？abbreviatus的假定的假想椎间盘生长因子（IDGF）具有显着的序列同源性。

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