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首页> 外文期刊>African Journal of Biotechnology >Microbial removal of weathered hydrocarbons by well adapted-bacteria
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Microbial removal of weathered hydrocarbons by well adapted-bacteria

机译：适应性强的细菌对风化烃的微生物去除

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The effectiveness of bioremediation processes may be limited by the physical and chemical properties of the pollutant, such as availability, recalcitrance, concentration and weathering, among others. The aim of this work was to evaluate the removal of recalcitrant oil fractions (aliphatic-aromatic and asphaltenic fractions) from a weathered soil, by two bacteria adapted to a high concentration of oil hydrocarbons, isolated from a soil with a concentration of 227,000 mg of total petroleum hydrocarbons per kg soil. Kinetics of hydrocarbons removal by?Bacillus coagulansand/or?Serratia liquefasciens?was performed in liquid culture for 168 h; hydrocarbons from soil as sole carbon and energy source (600 mg/l) were added and each of the microorganisms was inoculated for evaluation independently or as a mixed culture. The aromatic fraction was removed by?B. coagulans?at 330 mg/l; by?S. liquefasciensat 130 mg/l; and by both microorganisms at 360 mg/l. The asphaltenic fraction was removed by?B. coagulans?at 23 mg/l; by?S. liquefasciens?at?15 mg/l; and by both microorganisms at 34 mg/l. Chromatographic analysis of the aliphatic-aromatic fraction showed the presence of branched aliphatic C6 to C26, polyaromatic substituted compounds of two and three rings, and heteroaromatic compounds of dibenzothiphene type. The compounds that were removed from the aliphatic-aromatic fraction were of all types in the range of C6 to C13.
机译：生物修复过程的有效性可能会受到污染物的物理和化学特性（例如可用性，顽固性，浓度和风化等）的限制。这项工作的目的是评估由两种适应高浓度碳氢化合物的细菌从风化土壤中去除顽固油馏分（脂族-芳香族和沥青质馏分）的方法，该细菌是从土壤中分离出的227,000 mg每公斤土壤中的总石油烃。在液体培养中168小时进行凝结芽孢杆菌和/或液化粘质沙雷氏菌去除烃的动力学。加入土壤中的碳氢化合物作为唯一碳和能源（600 mg / l），并分别接种每种微生物以进行评估，也可以混合培养。用△B除去芳族馏分。凝集素？330 mg / l;由？S。液草素130 mg / l;两种微生物的剂量均为360 mg / l。用△B除去沥青质馏分。凝结物？23 mg / l;由？S。液化纤溶酶？15 mg / l;两种微生物的浓度均为34 mg / l。脂族-芳族部分的色谱分析表明存在分支的脂族C 6至C 26，两个和三个环的多芳族取代的化合物以及二苯并噻吩型的杂芳族化合物。从脂族-芳族馏分中除去的化合物是在C6至C13范围内的所有类型。

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