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首页> 外文期刊>African Journal of Biotechnology >Chitinolytic assay for Trichoderma species isolated from different geographical locations of Uttar Pradesh
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Chitinolytic assay for Trichoderma species isolated from different geographical locations of Uttar Pradesh

机译：从北方邦不同地理位置分离的木霉菌种的几丁质酶分析

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Chitin is the most commonly available polymer on the earth. Cell walls of most of the fungi are made up of chitin. As we all know that Trichoderma produces a wide variety of cell wall degrading enzymes (CWDEs) such as chitinase, xylanase, glucanase and cellulase. Out of these CWDEs chitinase is of prime importance as it is the building block of fungal cell walls. For the detection of chitinase activity we used a simple and sensitive method. We supplemented the chitinase detection media with coloidal chitin as a carbon source and bromocresol purple as pH indicator dye. This method is easy, sensitive, reproducible and economical. Colloidal chitin derived from sea-shells and commercial chitin is supplemented as carbon source in chitinase broth and solid media for the detection of chitinolytic and exochitinase activity. The chitinolytic activities were ranged from 6.2 to 3.9 and 4.8 to 1.8 mg/ml and exochitinase activities ranged from 0.0133 × 10-3 ?to 0.0076 × 10-3 and 0.00609 to 0.0055 × 10-3 U/ml), respectively, with colloidal chitin derived from commercial chitin and sea-shells.
机译：几丁质是地球上最常见的聚合物。大多数真菌的细胞壁由几丁质组成。众所周知，木霉产生多种细胞壁降解酶（CWDE），例如几丁质酶，木聚糖酶，葡聚糖酶和纤维素酶。在这些CWDE中，几丁质酶是最重要的，因为它是真菌细胞壁的基础。为了检测几丁质酶活性，我们使用了一种简单而灵敏的方法。我们用胶体甲壳质作为碳源和溴甲酚紫作为pH指示剂染料补充了几丁质酶检测介质。该方法简便，灵敏，可重复且经济。衍生自贝壳和商业甲壳质的胶质甲壳质作为甲壳素酶肉汤和固体培养基中的碳源被补充，用于检测甲壳质分解酶和Exochitinase活性。几丁质胶体的甲壳质分解活性范围为6.2至3.9和4.8至1.8 mg / ml，外切酶活性分别为0.0133×10-3？至0.0076×10-3和0.00609至0.0055×10-3 U / ml。甲壳质衍生自商业甲壳质和贝壳。

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