• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >Assessment of plantain (Musa sapientum L.) accessions genotypic groups relatedness using simple sequence repeats markers
【24h】

Assessment of plantain (Musa sapientum L.) accessions genotypic groups relatedness using simple sequence repeats markers

机译：使用简单的序列重复标记评估车前草（Musa sapientum L.）种质基因型群体的相关性

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Plantains are important sources of high-calorie energy in Ghana. They are also of great socio-economic importance in the country, and very important sources of rural income. Although several species exist all over the world, plantains belonging to the AAB group are unique to West Africa and Ghanaian collections have unique features and peculiar taste. Morphological and biochemical characterization are the popular techniques used to characterize plantain genotypes in Ghana. Thus, there is limited report on molecular characterization of plantains genotypes. Characterization based on morphologic characteristics alone may be limited since the expression of quantitative traits is subjective to strong environmental influence. Alternatively, molecular characterization techniques are capable of identifying polymorphism represented by differences in DNA sequences. The objective of this research was therefore to conduct molecular characterization of Ghanaian local accessions of plantain and assess relationship amongst known genotypic groups (populations). This study sampled 40 accessions of plantains representing four popular genotypic groups. Simple Sequence Repeats (SSRs) were used to assess diversity in reference to a set of global Musa collections. The 40 accessions of plantain were clustered into populations as being French plantain, True Horn, False Horn, and Hybrid prior to analysis. PopGene version 32 was used to analyze the data. This revealed that the overall plantain population used have Shannon’s Informative Index (I) value of 0.61±0.28 in the overall plantain population,? 100% polymorphism for all loci, 2.7±0.67 and 1.81±0.45 for ne and na respectively. Average heterozygosity was 0.34±0.17, loci mMaCIR231 and mMaCIR07 were the most informative, having I values of 0.85 and 0.81 respectively. The Fis and Fit values were both negative indicating lack of inbreeding and the gene flow value was 0.533. The study also revealed relationship among the various populations (French plantain, True Horn, False Horn, and Hybrid) on basis of molecular characterization.
机译：车前草是加纳高热量能源的重要来源。它们在该国也具有重要的社会经济意义，是农村收入的重要来源。尽管全世界有几种物种，但属于AAB组的车前草在西非是独特的，加纳的收藏品具有独特的特征和独特的味道。形态学和生化表征是用于表征加纳车前草基因型的流行技术。因此，关于车前草基因型的分子表征的报道有限。由于定量特征的表达受强烈的环境影响，因此仅基于形态特征的表征可能会受到限制。可选择地，分子表征技术能够鉴定由DNA序列差异代表的多态性。因此，本研究的目的是对车前草的加纳地方种质进行分子鉴定，并评估已知基因型群（种群）之间的关系。这项研究从40个车前草样品中取样，代表了四个流行的基因型群体。简单序列重复（SSR）用于参考一组全球Musa集合来评估多样性。在分析之前，将40种车前草种群分为法国车前草，真角，假角和杂种。 PopGene版本32用于分析数据。这表明所使用的整个车前草种群中香农的信息量指数（I）值在整个车前草种群中为0.61±0.28，所有基因座的100％多态性，ne和na分别为2.7±0.67和1.81±0.45。平均杂合度为0.34±0.17，位点mMaCIR231和mMaCIR07提供的信息最多，I值分别为0.85和0.81。 Fis和Fit值均为负，表明没有近交，基因流值为0.533。该研究还根据分子特征揭示了不同种群（法国车前草，真角，假角和杂种）之间的关系。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号