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首页> 外文期刊>African Journal of Biotechnology >Presence of Bacillus thuringiensis (Bt) gene in cereals and cereal-based products in local markets and supermarkets of Yaound, Cameroon
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Presence of Bacillus thuringiensis (Bt) gene in cereals and cereal-based products in local markets and supermarkets of Yaound, Cameroon

机译：在喀麦隆雅温得的当地市场和超市中，谷物和谷物产品中存在苏云金芽孢杆菌（Bt）基因

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摘要

The use and importation of genetically modified (GM) crops and products derived from these crops are regulated by national and international policies, which unfortunately are often not properly implemented in some countries. Given the ongoing globalization of trade and increasing availability of GM plant products, countries like Cameroon with a weak system to regulate the importation of these products face the threat of these products entering local markets. This study investigated the presence of GM cereals and cereal-based products circulating in the local markets and supermarkets in Yaoundé, Cameroon. An inventory of cereal based products from these markets was conducted and one of the products was labeled as being derived from GM cereals crops. DNA was extracted from 26 products with a protocol using SDS guanidine thiocyanate to assess the presence of the Bacillus thuringiensis (Bt) gene. Polymerase chain reaction (PCR) was used to amplify the DNA fragment associated with Bt gene. Majority of the products were maize-soya based and wheat-soya based. The Bt gene was present in four of the 14 maize based products tested. The presence of the Bt gene in these cereal based products suggest the need for these products to be labeled according to international regulations.
机译：转基因作物及其衍生产品的使用和进口受到国家和国际政策的管制，不幸的是，在某些国家中，这些政策往往得不到适当的实施。鉴于贸易的持续全球化和转基因植物产品的可用性不断提高，喀麦隆这样的监管这些产品进口体系薄弱的国家面临着这些产品进入本地市场的威胁。这项研究调查了在喀麦隆雅温得的当地市场和超市中流通的转基因谷物和谷物产品的存在。对这些市场的谷类产品进行了盘点，其中一种产品被标记为来自转基因谷类作物。使用SDS硫氰酸胍从方案中从26种产品中提取DNA，以评估苏云金芽孢杆菌（Bt）基因的存在。聚合酶链反应（PCR）用于扩增与Bt基因相关的DNA片段。产品的大多数是基于玉米-大豆和基于小麦-大豆的。在测试的14种基于玉米的产品中，有4种存在Bt基因。这些谷物类产品中Bt基因的存在表明需要根据国际法规对这些产品进行标记。

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