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首页> 外文期刊>African Journal of Biotechnology >Functional analysis of a gene encoding threonine synthase from rice
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Functional analysis of a gene encoding threonine synthase from rice

机译：水稻苏氨酸合酶编码基因的功能分析

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摘要

Threonine synthase (TS) is a pyridoxal phosphate dependent enzyme that catalyzes the formation of threonine (Thr) through?O-phosphohomoserine (OPH) from the aspartate family pathway in plants. The properties of the TS enzyme have been evaluated in many bacteria and few plants. Sequence analysis of the cDNA from rice revealed that it harbors a full-length open reading frame for?OsTSencoding for 521 amino acids, corresponding to a protein of approximately 57.2 kD. The predicted amino acid sequence of OsTS?is highly homologous to that ofArabidopsis?TS and many bacterial TS encoded by?thrC?gene. The OsTS protein harbors a signature binding motif for pyridoxal- 5’ -phosphate at the amino terminus. A?thrC?mutant strain of?Escherichia coli?was complemented by?OsTS?expression.OsTS?expression was correlated with the survival of the?thrC?mutant, which is affected by the supplementation of an aspartate pathway metabolite, methionine.
机译：苏氨酸合酶（TS）是磷酸吡ido醛依赖性酶，可通过植物的天冬氨酸家族途径通过αO-磷酸高丝氨酸（OPH）催化苏氨酸（Thr）的形成。已经在许多细菌和少量植物中评估了TS酶的特性。水稻cDNA的序列分析表明，它具有一个全长的开放阅读框，用于编码521个氨基酸的ΔOsTS，相当于一个约57.2 kD的蛋白质。 OsTSα的预测氨基酸序列与拟南芥αTS和许多由thrCα基因编码的细菌TS高度同源。 OsTS蛋白在氨基末端带有一个吡ido醛5'-磷酸的签名结合基序。大肠杆菌的一个thrC突变株补充有OsTS的表达。OsTS的表达与thrC突变的存活有关，这受天冬氨酸途径代谢产物蛋氨酸的补充的影响。

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