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首页> 外文期刊>African Journal of Biotechnology >Natural genetic variation in Calligonum Tunisian genus analyzed by RAPD markers
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Natural genetic variation in Calligonum Tunisian genus analyzed by RAPD markers

机译：用RAPD标记分析突刺Call属的自然遗传变异。

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The?Calligonum?genus is one of the most economically important resources of the Tunisian desert, playing an important role in the lives of desert local population. A great range of genetic diversity could be seen in diverse populations of this genus which are spread all over Tunisian areas. DNA-based molecular markers are playing increasingly important role in the analysis of genetic diversity in wide range of plants. This?study is an attempt to collect, compile and collate information on the existing natural genetic diversity, at intra-specific and inter-populational levels inCalligonum?genus (C.azel,?C.arich?and?C.comosum). This?study?deals with 16 genotypes of?Calligonum?(Polygonaceae) collected from different regions of Tunisian desert using random amplified polymorphic DNA (RAPD) marker. In total,46 polymorphic bands amplified by 4 random primers, with the polymorphic rate of 89.06%?were?recorded.?The commercial software package SPSS 16?was?used to develop similar matrices based on the Dice coefficient which is defined as 2a/2a+u, where “a” is the number of positive matches and “u” is the number of non-matches.From the analysis, it is imperative that predominantly obligate out breeding behavior of?Calligonum?genus, helps these diverse accessions to spread and occupy specific geographical niches in the Tunisian arid regions.
机译：Call属是突尼斯沙漠中最重要的经济资源之一，在沙漠当地居民的生活中起着重要的作用。在该属的不同种群中可见到广泛的遗传多样性，这些种群遍布突尼斯地区。基于DNA的分子标记在分析多种植物的遗传多样性中起着越来越重要的作用。本研究旨在收集，整理和整理有关Calligonum？属（C.azel，C.arich？和C.comosum）种内和种群间水平的现有自然遗传多样性信息。这项研究使用随机扩增多态性DNA（RAPD）标记从突尼斯沙漠不同地区收集的16种Calligonum（Polygonaceae）基因型进行了研究。总共记录了4条随机引物扩增出的46条多态性条带，多态性率为89.06％？。使用商业软件包SPSS 16 ?，根据Dice系数定义为2a /，开发相似的矩阵。 2a + u，其中“ a”是阳性匹配的数目，而“ u”是不匹配的数目。从分析来看，至关重要的是，必须专心致力于“ Calligonum”属的繁殖行为，以帮助这些多样的种质加入在突尼斯干旱地区传播和占领特定的地理生态位。

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