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首页> 外文期刊>African Journal of Biotechnology >Optimization of ultrasound-assisted extraction of total phenol from betel (Areca catechu L.) nut seed and evaluation of antioxidant activity in vitro
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Optimization of ultrasound-assisted extraction of total phenol from betel (Areca catechu L.) nut seed and evaluation of antioxidant activity in vitro

机译：超声波辅助从槟榔种子中提取总酚的优化及体外抗氧化活性的评价

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摘要

Optimization of the ultrasound-assisted extraction technology for total phenol from betel?(Areca catechu?L.)?nut seed was carried out. On the basis of one factor tests, the method of response surface analysis with 3 factors including extracting temperature, time and solvent-material ratio on the content of total phenol was adopted. The optimal extracting conditions are as follows: extraction temperature 58°C, extraction time 42 min and solvent-material ratio 53 ml/g. The predicted value and measured value of total phenol?was?164.74 mg catechin equivalents/g of betel nut seed (mg CE/g BNS) and 160.95 mg CE/g BNS under this condition, respectively. The results indicate that the obtained model developed by response surface methodology is feasible for practical prediction. The experiments of antioxidant activity showed?that the betel nut seed extract presents the strong antioxidant activities to the DPPH and ABTS radical and the EC50?is 113.42 and 105.69 μg/ml, respectively.
机译：超声波辅助提取槟榔（槟榔）种子总酚的工艺优化。在单因素试验的基础上，采用提取温度，萃取时间，溶剂-原料比对总酚含量三因素进行响应面分析的方法。最佳提取条件如下：提取温度58℃，提取时间42分钟，溶剂-物料比53 ml / g。在此条件下，总酚δ的预测值和实测值分别为164.74 mg儿茶素当量/ g槟榔籽（mg CE / g BNS）和160.95 mg CE / g BNS。结果表明，利用响应面法建立的模型可用于实际预测。抗氧化活性实验表明，槟榔籽提取物对DPPH和ABTS自由基具有较强的抗氧化活性，EC50分别为113.42和105.69μg/ ml。

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