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首页> 外文期刊>African Journal of Biotechnology >Effect of alternating and direct currents on Pseudomonas aeruginosa growth in vitro
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Effect of alternating and direct currents on Pseudomonas aeruginosa growth in vitro

机译：交流电和直流电对铜绿假单胞菌体外生长的影响

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Eradication of pathogenic bacteria from important part of our life such as dental tools, foods and wounds is necessary. Based on the effect of natural selection, these bacteria become resistant to antibiotics. In some cases such as the section where burnt are treated in the hospital, we observe high rate of mortality as well as high numbers of resistant bacteria. In order to solve these problems, electrical stimulation (ES) is proposed. This has being shown to be an effective method. One of the reasons why it works could be due to the bacteria static property of electrical stimulation. So, more studies must be done so as to reach optimum voltage and currents. The test media were Muller-Hinton agar and eosin methylene blue (EMB) agar. In this research?Pseudomonas aeruginosa?which was isolated from patients? wounds was examined with levels of alternating and direct current (AC and DC) electrical stimulation (1.5V, 3.5V, 5.5V and 10V) to see if these currents could inhibitP. aeruginosa?growth in vitro. The experiment was performed in two forms: The first was carried out immediately while the second was carried out 19 h after being cultured. Different patterns of zone of inhibition were observed in the two forms of our research. AC current had low inhibitive effect on?P. aeruginosa?s?growth. Anode and cathode showed different zone of inhibition, in each of the forms and media. The maximum inhibition zone (22 mm) was observed around cathode in 3.5 V direct current which was immediately used in the media. Direct current significantly inhibits growth of?P. aeruginosa. Based on other studies on different bacterial species, ES can be applied to sterilization and controlling of superficial infections like in burnt patients.
机译：必须从我们生活的重要部分中消除病原细菌，例如牙科工具，食物和伤口。基于自然选择的作用，这些细菌对抗生素具有抗性。在某些情况下，例如医院烧伤治疗的部分，我们观察到高死亡率和高耐药菌数。为了解决这些问题，提出了电刺激（ES）。已经证明这是一种有效的方法。它起作用的原因之一可能是由于电刺激的细菌静态特性。因此，必须进行更多的研究才能达到最佳的电压和电流。测试培养基是穆勒-欣顿琼脂和曙红亚甲基蓝（EMB）琼脂。在这项研究中，铜绿假单胞菌是从患者身上分离出来的吗？用交流电和直流电（AC和DC）（1.5V，3.5V，5.5V和10V）的电刺激水平检查伤口，看这些电流是否可以抑制P。铜绿菌在体外生长。实验以两种形式进行：第一种立即进行，第二种在培养后19小时进行。在我们的两种研究形式中观察到不同的抑制区模式。交流电流对ΔP的抑制作用低。铜绿假单胞菌的生长。阳极和阴极在每种形式和介质中均显示出不同的抑制区。在3.5 V直流电下，在阴极周围观察到最大抑制区（22 mm），该电流立即用于介质中。直流电显着抑制了？P的生长。铜绿。根据对不同细菌种类的其他研究，ES可用于绝育和控制浅表感染，例如在烧伤患者中。

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