RNAi technology extends its reach: Engineering plant resistance against harmful eukaryotes

Jun-Hai Niu; Heng Jian; Jian-Mei Xu; Yang-Dong Guo; Qian Liu

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RNAi technology extends its reach: Engineering plant resistance against harmful eukaryotes

机译：RNAi技术扩展了其范围：工程植物对有害真核生物的抗性

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RNA interference (RNAi) is a homology-dependent gene silencing technology that is initiated by double stranded RNA (dsRNA). It has emerged as a genetic tool for engineering plants resistance against prokaryotic pathogens such as virus and bacteria. Recent studies broaden the role of RNAi, and many successful examples have described the application of RNAi for engineering plant resistance against a range of eukaryotic organisms. Expression of dsRNA directed against suitable eukaryotic pathogens target genes in transgenic plants has been shown to give protection against harmful eukaryotic species, including nematodes, herbivorous insects, parasitic weeds and fungi. This review addresses the progress of RNAi-based transgenic plant resistance against these four class eukaryotic pests, as well as future challenges and prospects.

机译：RNA干扰（RNAi）是一种依赖同源性的基因沉默技术，由双链RNA（dsRNA）引发。它已成为工程植物抗原核病原体如病毒和细菌的遗传工具。最近的研究拓宽了RNAi的作用，许多成功的例子描述了RNAi在工程植物对一系列真核生物的抗性中的应用。已经表明针对转基因植物中针对合适的真核病原体靶基因的dsRNA的表达可提供针对有害真核生物的保护，包括线虫，草食性昆虫，寄生性杂草和真菌。这项审查解决了基于RNAi的转基因植物对这四类真核生物害虫的抗性的进展，以及未来的挑战和前景。

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《African Journal of Biotechnology》 |2010年第45期|共10页
作者
Jun-Hai Niu; Heng Jian; Jian-Mei Xu; Yang-Dong Guo; Qian Liu;
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中图分类生物工程学（生物技术）;
关键词
dsRNARNAicrop resistancebiotechnologynematodeinsectparasitic weedfungus;

机译：dsRNARNA抗农生物技术线虫昆虫寄生杂草真菌;

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1. RNAi technology extends its reach: Engineering plant resistance against harmful eukaryotes [J] . Jun-Hai Niu, Heng Jian, Jian-Mei Xu, African Journal of Biotechnology . 2010,第45期

机译：RNAi技术扩展了其范围：工程植物对有害真核生物的抗性
2. Engineering broad root-knot resistance in transgenic plants by RNAi silencing of a conserved and essential root-knot nematode parasitism gene [J] . Guozhong Huang, Rex Allen, Eric L. Davis, Proceedings of the National Academy of Sciences of the United States of America . 2006,第39期

机译：通过保守和必要的根结线虫寄生基因的RNAi沉默工程化转基因植物中广泛的根结抗性
3. Increasing the Resistance of Rape Plants to the Parasitic Nematode Heterodera schachtii Using RNAi Technology [J] . V. A. Tsygankova, A. I. Yemets, H. O. Iutinska, Cytology and genetics . 2013,第4期

机译：利用RNAi技术提高油菜对寄生线虫Heterodera schachtii的抗性
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5. Molecular characterizations of transgenic Nicotiana benthamiana plants resistant to Red clover necrotic mosaic virus and effects of mixed infections with Potato virus Y on RNAi-mediated resistance [D] . Solofoharivelo, Marie Chrystine 2008

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6. Engineering broad root-knot resistance in transgenic plants by RNAi silencing of a conserved and essential root-knot nematode parasitism gene [O] . Guozhong Huang, Rex Allen, Eric L. Davis, 2006

机译：通过保守和必要的根结线虫寄生基因的RNAi沉默工程化转基因植物中广泛的根结抗性
7. Engineering broad root-knot resistance in transgenic plants by RNAi silencing of a conserved and essential root-knot nematode parasitism gene [O] . Huang, Guozhong, Allen, Rex, Davis, Eric L., 2006

机译：通过保守和必不可少的根结线虫寄生基因的RNAi沉默工程化转基因植物中广泛的根结抗性
8. Research and Technology 1995: Annual Report of the Marshall Space Flight Center.Technology for the Stars: Extending Our Reach [R] . 1995

机译：1995年研究与技术：马歇尔太空飞行中心年度报告。明星技术：扩展我们的影响力

RNAi technology extends its reach: Engineering plant resistance against harmful eukaryotes

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