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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Engineering broad root-knot resistance in transgenic plants by RNAi silencing of a conserved and essential root-knot nematode parasitism gene
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Engineering broad root-knot resistance in transgenic plants by RNAi silencing of a conserved and essential root-knot nematode parasitism gene

机译：通过保守和必要的根结线虫寄生基因的RNAi沉默工程化转基因植物中广泛的根结抗性

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摘要

Secreted parasitism proteins encoded by parasitism genes expressed in esophageal gland cells mediate infection and parasitism of plants by root-knot nematodes (RKN). Parasitism gene 16D10 encodes a conserved RKN secretory peptide that stimulates root growth and functions as a ligand for a putative plant transcription factor. We used in vitro and in vivo RNA interference approaches to silence this parasitism gene in RKN and validate that the parasitism gene has an essential function in RKN parasitism of plants. Ingestion of 16D10 dsRNA in vitro silenced the target parasitism gene in RKN and resulted in reduced nematode infec-tivity. In vivo expression of 16D10 dsRNA in Arabidopsis resulted in resistance effective against the four major RKN species. Because no known natural resistance gene has this wide effective range of RKN resistance, bioengineering crops expressing dsRNA that silence target RKN parasitism genes to disrupt the parasitic process represents a viable and flexible means of developing novel durable RKN-resistant crops and could provide crops with unprecedented broad resistance to RKN.
机译：由食管腺细胞中表达的寄生基因编码的分泌的寄生蛋白介导根结线虫（RKN）侵染和寄生植物。寄生病毒基因16D10编码一个保守的RKN分泌肽，该肽能刺激根部生长并充当推定植物转录因子的配体。我们使用了体外和体内RNA干扰方法来沉默RKN中的该寄生基因，并验证该寄生基因在植物的RKN寄生中具有必不可少的功能。体外摄入16D10 dsRNA使RKN中的目标寄生基因沉默，并导致线虫感染力降低。在拟南芥中体内表达16D10 dsRNA导致有效抵抗四种主要RKN物种。因为没有已知的天然抗性基因具有如此广泛的RKN抗性范围，所以表达dsRNA的生物工程农作物沉默目标RKN寄生基因以破坏寄生过程，这是开发新型耐RKN耐久农作物的可行且灵活的方法，可以为农作物提供前所未有的抗性。对RKN的广泛抵抗。

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