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首页> 外文期刊>African Journal of Biotechnology >Development of a sequence characterized amplified region (SCAR) marker for identification of Thai jasmine rice (Oryza sativa L.) variety KDML105
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Development of a sequence characterized amplified region (SCAR) marker for identification of Thai jasmine rice (Oryza sativa L.) variety KDML105

机译：开发用于鉴定泰国茉莉花水稻（Oryza sativa L.）品种KDML105的序列特征扩增区（SCAR）标记

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摘要

The varieties of rice are difficult to be distinguished because of their similar morphological characters, therefore rice variety identification or differentiation is very important. The authentication by?using molecular marker or DNA fingerprinting is the most accurate method. In this study, random amplified polymorphic DNA (RAPD) derived sequence characterized amplified region (SCAR) markers were applied to detect aromatic rice (Oryza sativa?L.) variety Khao Dawk Mali (KDML) 105 from its closely related varieties. Eighty 10-mer primers were screened to identify an OPJ01KDML105-727?band which was amplified by primer OPJ-01, and this band specified to variety KDML105. SCAR marker was created by cloning and sequencing the specific band, and designing a pair of primers, SCJ01KDML105-668?to amplify the band of 668 bp. Diagnostic PCRs were performed using the primer pair with the total DNAs of?O. sativa?var. KDML105, and its related varieties as templates, with only?O. sativa?var. KDML105 being able to be amplified. This amplification is not only rapid, but is also easy to perform. Hence,?it is a feasible method for identifying?O. sativa?var. KDML105 between rice varieties.
机译：水稻品种由于其相似的形态特征而难以区分，因此水稻品种的鉴定或分化非常重要。通过使用分子标记或DNA指纹进行身份验证是最准确的方法。在这项研究中，随机扩增多态性DNA（RAPD）衍生的序列特征性扩增区（SCAR）标记被用于检测香稻（Krydawk Mali）（KDML）105的密切相关品种。筛选了八十个10聚体引物，以鉴定由引物OPJ-01扩增的OPJ01KDML105-727Δ条带，该条带被指定用于KDML105变种。通过克隆和测序特定条带，并设计一对引物SCJ01KDML105-668 ?，以扩增668 bp的条带来创建SCAR标记。使用引物对和总DNA的O进行诊断PCR。苜蓿KDML105及其相关变体作为模板，只有？O。苜蓿KDML105能够被放大。这种放大不仅快速，而且易于执行。因此，这是一种确定O的可行方法。苜蓿水稻品种之间的KDML105。

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